Dorfin Polyclonal Antibody
Ucallm_WP12058-50
Price range: $268.00 through $328.00
Product details
Background:
This gene encodes a member of the ring between ring fingers (RBR) protein family, and the encoded protein contains two RING-finger motifs and an in between RING fingers motif. This protein is an E3 ubiquitin ligase that is localized to Lewy bodies, and ubiquitylates synphilin-1, which is an interacting protein of alpha synuclein in neurons. The encoded protein may be involved in amyotrophic lateral sclerosis and Parkinson's disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013],
Specifications
| Size | 50μL/100μL |
| Target | Dorfin |
| Reactivity | Human, Mouse |
| Applications | WB, ELISA |
| MW(Calculated) | |
| MW(Observed) | 90kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; ELISA 1:40000; Not yet tested in other applications. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human RNF19A. AA range:51-100 |
| Specificity | Dorfin Polyclonal Antibody detects endogenous levels of Dorfin protein. |
| Gene Name | RNF19A |
| Protein Name | E3 ubiquitin-protein ligase RNF19A |
| Other Name | RNF19A; / RNF19; / E3 ubiquitin-protein ligase RNF19A; / Double ring-finger protein; / Dorfin; / RING finger protein 19A; / p38 |
| Species | Human |
| Gene ID | 25897 |
| Uniprot | Q9NV58, |
| Species.1 | Mouse |
| Gene ID.1 | 30945 |
| Uniprot.1 | P50636 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Membrane; Multi-pass membrane protein . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Present in the hyaline inclusion bodies specifically found in motor neurons from amyotrophic lateral sclerosis patients. Present in the Lewy bodies specifically found in neurons from Parkinson disease patients. |
| Tissue Expression | Widely expressed, with highest levels in heart. Ubiquitously expressed in the central nervous system. |
| Signaling_pathway | |
| Research Areas | |
| Function | Function:E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates, such as SNCAIP or CASR. Specifically ubiquitinates pathogenic SOD1 variants, which leads to their proteasomal degradation and to neuronal protection.,pathway:Protein modification; protein ubiquitination.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the RBR family. RNF19 subfamily.,similarity:Contains 1 IBR-type zinc finger.,similarity:Contains 2 RING-type zinc fingers.,subcellular location:Present in the hyaline inclusion bodies specifically found in motor neurons from amyotrophic lateral sclerosis patients. Present in the Lewy bodies specifically found in neurons from Parkinson disease patients.,subunit:Interacts with UBE2L3 and UBE2L6. Interacts with transcription factor Sp1. Interacts with VCP, CASR, SNCAIP and with some SOD1 variants which cause amyotrophic lateral sclerosis, but not with wild-type SOD1.,tissue specificity:Widely expressed, with highest levels in heart. Ubiquitously expressed in the central nervous system., |
Please login to post questions
Related products
