Price range: $268.00 through $328.00
Background:
The protein encoded by this gene is a histone methyltransferase that methylates lysine-79 of histone H3. It is inactive against free core histones, but shows significant histone methyltransferase activity against nucleosomes. [provided by RefSeq, Aug 2011],
| Size | 50μL/100μL |
| Target | DOT1L |
| Reactivity | Human |
| Applications | WB, ELISA |
| MW(Calculated) | |
| MW(Observed) | 191kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-2000; ELISA 1:5000-20000 |
| Formulation | Liquid in PBS containing 50% glycerol,0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthesized peptide derived from human protein . at AA range: 700-780 |
| Specificity | DOT1L Polyclonal Antibody detects endogenous levels of protein. |
| Gene Name | DOT1L KIAA1814 KMT4 |
| Protein Name | Histone-lysine N-methyltransferase, H3 lysine-79 specific (DOT1-like protein) (Histone H3-K79 methyltransferase) (H3-K79-HMTase) (Lysine N-methyltransferase 4) |
| Other Name | |
| Species | Human |
| Gene ID | 84444 |
| Uniprot | Q8TEK3 |
| Species.1 | |
| Gene ID.1 | |
| Uniprot.1 | |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nucleus . |
| Tissue Expression | Brain,Epithelium,Spleen, |
| Signaling_pathway | Human Diseases >> Cancer: overview >> Transcriptional misregulation in cancer |
| Research Areas | >>Lysine degradation; / >>Metabolic pathways; / >>Transcriptional misregulation in cancer |
| Function | Catalytic activity:S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N(6)-methyl-L-lysine.,Function:Histone methyltransferase. Methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones. Binds to DNA.,miscellaneous:In contrast to other lysine histone methyltransferase, it does not contain a SET domain, suggesting the existence of another mechanism for methylation of lysine residues of histones.,similarity:Belongs to the DOT1 family., |
