Drosophila Melanogaster β-actin Mouse mAb [Mix-mA]
Product details
Background:
β-actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, antibodies against β-actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-actin may not be stable in certain cells. For example, expression of β-actin in adipose tissue is very low and therefore β-actin should not be used as loading control for these tissues.
Specifications
| Size | 50μL/100μL |
| Target | Actin β |
| Reactivity | Drosophila, Human, Mouse, Rat |
| Applications | IHC, WB |
| MW(Calculated) | |
| MW(Observed) | 42kD |
| Host Species | Mouse |
| Isotype | |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC1:50-200; WB 1:1000-2000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Monoclonal |
| Clone Number | 15H5 |
| Immunogen | Synthesized peptide derived from human Drosophila Melanogaster β-actin |
| Specificity | This antibody detects endogenous levels of Drosophila Melanogaster β-actin at Human, Mouse,Rat |
| Gene Name | Drosophila Melanogaster β-actin |
| Protein Name | Drosophila Melanogaster β-actin |
| Other Name | |
| Species | Human |
| Gene ID | |
| Uniprot | P10987 |
| Species.1 | |
| Gene ID.1 | |
| Uniprot.1 | |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | |
| Tissue Expression | |
| Signaling_pathway | |
| Research Areas | |
| Function |
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