Name: E-cadherin Monoclonal Antibody
SKU: WM0150
Price: 268 USD
Availability: InStock

Product details

Background:
This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16. [provided by RefSeq, Nov 2015],

Specifications

Size	50μL/100μL
Target	E-cadherin
Reactivity	Human, Mouse, Monkey
Applications	WB, IHC, IF, FC, ELISA
MW(Calculated)
MW(Observed)	125-130kD
Host Species	Mouse
Isotype
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	WB 1:500-1:2000; IHC 1:200-1:1000; Flow Cyt 1:200-1:400; ELISA 1:10000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification	Affinity purification
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	18B12
Immunogen	Purified recombinant fragment of human E-cadherin expressed in E. Coli.
Specificity	E-cadherin Monoclonal Antibody detects endogenous levels of E-cadherin protein.
Gene Name	CDH1
Protein Name	Cadherin-1
Other Name	CDH1; / CDHE; / UVO; / Cadherin-1; / CAM 120/80; / Epithelial cadherin; / E-cadherin; / Uvomorulin; / CD antigen CD324
Species	Human
Gene ID	999
Uniprot	P12830,
Species.1	Mouse
Gene ID.1	12550
Uniprot.1	P09803
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Cell junction, adherens junction . Cell membrane; Single-pass type I membrane protein. Endosome. Golgi apparatus, trans-Golgi network. Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane.
Tissue Expression	Non-neural epithelial tissues.
Signaling_pathway	Cellular Processes >> Cellular community - eukaryotes >> Adherens junction;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: specific types >> Gastric cancer;Human Diseases >> Cancer: specific types >> Thyroid cancer;Human Diseases >> Cancer: specific types >> Melanoma;Human Diseases >> Cancer: specific types >> Bladder cancer;Human Diseases >> Cancer: specific types >> Endometrial cancer;Environmental Information Processing >> Signal transduction >> Rap1 signaling pathway;Environmental Information Processing >> Signal transduction >> Hippo signaling pathway;Environmental Information Processing >> Signal transduction >> Apelin signaling pathway;Environmental Information Processing >> Signaling molecules and interaction >> Cell adhesion molecules
Research Areas	>>Rap1 signaling pathway; / >>Apelin signaling pathway; / >>Hippo signaling pathway; / >>Cell adhesion molecules; / >>Adherens junction; / >>Bacterial invasion of epithelial cells; / >>Pathways in cancer; / >>Endometrial cancer; / >>Thyroid cancer; / >>Melanoma; / >>Bladder cancer; / >>Gastric cancer
Function	Disease:Defects in CDH1 are a cause of gastric cancer [MIM:137215]; also known as hereditary familial diffuse gastric cancer (HDGC).,Disease:Defects in CDH1 are a cause of susceptibility to endometrial cancer [MIM:608089].,Disease:Defects in CDH1 are associated with ovarian cancer [MIM:167000]. Ovarian cancer is the leading cause of death from gynecologic malignancy. It is characterized by advanced presentation with loco-regional dissemination in the peritoneal cavity and the rare incidence of visceral metastases. These typical features relate to the biology of the disease, which is a principal determinant of outcome.,Disease:Defects in CDH1 are involved in dysfunction of the cell-cell adhesion system, triggering cancer invasion (gastric, breast, ovary, endometrium and thyroid) and metastasis.,Function:Cadherins are calcium dependent cell adhesion proteins.,Function:Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.,Function:E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.,online information:E-cadherin entry,PTM:During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3 to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disaaaembly of adherens junctions.,similarity:Contains 5 cadherin domains.,subcellular location:Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm.,subunit:Homodimer; disulfide-linked. Interacts directly, via the cytoplasmic domain, with CTNNB1 or JUP to form the PSEN1/cadherin/catenin adhesion complex which connects to the actin skeleton through the actin binding of alpha-catenin. Interaction with PSEN1, cleaves CDH1 resulting in the disassociation of cadherin-based adherens junctions (CAJs). Interacts with AJAP1, CTNND1 and DLGAP5.,tissue specificity:Non-neural epithelial tissues.,

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E-cadherin Monoclonal Antibody

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E-cadherin Monoclonal Antibody

Product details

Specifications

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