Price range: $268.00 through $328.00
Background:
The protein encoded by this gene is structurally similar to G protein-coupled receptors and is highly expressed in endothelial cells. It binds the ligand sphingosine-1-phosphate with high affinity and high specificity, and suggested to be involved in the processes that regulate the differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016],
| Size | 50μL/100μL |
| Target | EDG-1 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 43kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:20000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human EDG1. AA range:5-54 |
| Specificity | EDG-1 Polyclonal Antibody detects endogenous levels of EDG-1 protein. |
| Gene Name | S1PR1 |
| Protein Name | Sphingosine 1-phosphate receptor 1 |
| Other Name | S1PR1; / CHEDG1; / EDG1; / Sphingosine 1-phosphate receptor 1; / S1P receptor 1; / S1P1; / Endothelial differentiation G-protein coupled receptor 1; / Sphingosine 1-phosphate receptor Edg-1; / S1P receptor Edg-1; / CD antigen CD363 |
| Species | Human |
| Gene ID | 1901 |
| Uniprot | P21453, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | O08530 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. Endosome. Membrane raft. Recruited to caveolin-enriched plasma membrane microdomains in response to oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine. Ligand binding leads to receptor internalization. |
| Tissue Expression | Endothelial cells, and to a lesser extent, in vascular smooth muscle cells, fibroblasts, melanocytes, and cells of epithelioid origin. |
| Signaling_pathway | Environmental Information Processing >> Signal transduction >> FoxO signaling pathway;Environmental Information Processing >> Signal transduction >> Sphingolipid signaling pathway |
| Research Areas | >>FoxO signaling pathway; / >>Sphingolipid signaling pathway; / >>Neuroactive ligand-receptor interaction |
| Function | Function:Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. This inducible epithelial cell G-protein-coupled receptor may be involved in the processes that regulate the differentiation of endothelial cells. Seems to be coupled to the G(i) subclass of heteromeric G proteins.,induction:By the tumor promoter phorbol 12-myristate 13-acetate (PME) in the presence of cycloheximide.,PTM:S1P-induced endothelial cell migration requires the PKB/AKT1-mediated phosphorylation of the third intracellular loop at the Thr-236 residue.,similarity:Belongs to the G-protein coupled receptor 1 family.,tissue specificity:Endothelial cells, and to a lesser extent, in vascular smooth muscle cells, fibroblasts, melanocytes, and cells of epithelioid origin., |
