Price range: $268.00 through $328.00
Background:
This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment. [provided by RefSeq, Jul 2008],
| Size | 50μL/100μL |
| Target | eEF2K |
| Reactivity | Human, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 105kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human eEF2K. AA range:331-380 |
| Specificity | eEF2K Polyclonal Antibody detects endogenous levels of eEF2K protein. |
| Gene Name | EEF2K |
| Protein Name | Eukaryotic elongation factor 2 kinase |
| Other Name | EEF2K; / Eukaryotic elongation factor 2 kinase; / eEF-2 kinase; / eEF-2K; / Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase |
| Species | Human |
| Gene ID | 29904 |
| Uniprot | O00418, |
| Species.1 | Mouse |
| Gene ID.1 | 13631 |
| Uniprot.1 | O08796, |
| Species.2 | Rat |
| Gene ID.2 | 25435 |
| Uniprot.2 | P70531 |
| Cellular Localization | cytoplasm,cytosol,postsynaptic density, |
| Tissue Expression | Epithelium,Glial tumor,Lymph,T-cell, |
| Signaling_pathway | Organismal Systems >> Endocrine system >> Oxytocin signaling pathway;Environmental Information Processing >> Signal transduction >> AMPK signaling pathway |
| Research Areas | >>AMPK signaling pathway; / >>Oxytocin signaling pathway |
| Function | Catalytic activity:ATP + [elongation factor 2] = ADP + [elongation factor 2] phosphate.,enzyme regulation:Undergoes calcium/calmodulin-dependent intramolecular autophosphorylation, and this results in it becoming partially calcium/calmodulin-independent.,Function:Phosphorylates eukaryotic elongation factor-2. Binds calmodulin.,similarity:Belongs to the protein kinase superfamily. Alpha-type protein kinase family.,similarity:Contains 1 alpha-type protein kinase domain.,subunit:Monomer or homodimer ., |
