EHMT1 Monoclonal Antibody
Ucallm_WM0153-50
Price range: $268.00 through $328.00
Product details
Background:
The protein encoded by this gene is a histone methyltransferase that is part of the E2F6 complex, which represses transcription. The encoded protein methylates the Lys-9 position of histone H3, which tags it for transcriptional repression. This protein may be involved in the silencing of MYC- and E2F-responsive genes and therefore could play a role in the G0/G1 cell cycle transition. Defects in this gene are a cause of chromosome 9q subtelomeric deletion syndrome (9q-syndrome, also known as Kleefstra syndrome). Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014],
Specifications
| Size | 50μL/100μL |
| Target | EHMT1 |
| Reactivity | Human |
| Applications | WB, ELISA |
| MW(Calculated) | 141kD |
| MW(Observed) | |
| Host Species | Mouse |
| Isotype | |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; ELISA 1:10000; Not yet tested in other applications. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | Affinity purification |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | 6D12 |
| Immunogen | Purified recombinant fragment of EHMT1 expressed in E. Coli. |
| Specificity | EHMT1 Monoclonal Antibody detects endogenous levels of EHMT1 protein. |
| Gene Name | EHMT1 |
| Protein Name | Histone-lysine N-methyltransferase, H3 lysine-9 specific 5 |
| Other Name | EHMT1; / EUHMTASE1; / GLP; / KIAA1876; / KMT1D; / Histone-lysine N-methyltransferase EHMT1; / Euchromatic histone-lysine N-methyltransferase 1; / Eu-HMTase1; / G9a-like protein 1; / GLP; / GLP1; / Histone H3-K9 methyltransferase 5; / H3-K9-HMTase 5; / Lysine N-methy |
| Species | Human |
| Gene ID | 79813 |
| Uniprot | Q9H9B1, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q5DW34 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nucleus. Chromosome. Associates with euchromatic regions. |
| Tissue Expression | Widely expressed. |
| Signaling_pathway | Organismal Systems >> Aging >> Longevity regulating pathway |
| Research Areas | >>Lysine degradation; / >>Metabolic pathways; / >>Longevity regulating pathway |
| Function | Alternative products:Experimental confirmation may be lacking for some isoforms,Catalytic activity:S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N(6)-methyl-L-lysine.,Disease:Defects in EHMT1 are the cause of chromosome 9q subtelomeric deletion syndrome (9q- syndrome) [MIM:610253]. Common features seen in these patients are severe mental retardation, hypotonia, brachy(micro)cephaly, epileptic seizures, flat face with hypertelorism, synophrys, anteverted nares, cupid bow or tented upper lip, everted lower lip, prognathism, macroglossia, conotruncal heart defects, and behavioral problems.,Domain:The SET domain mediates interaction with WIZ.,Function:Histone methyltransferase. Methylates 'Lys-9' of histone H3 (in vitro). H3 'Lys-9' methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in G0/G1 transition in cell cycle.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,sequence Caution:Intron retention.,similarity:Belongs to the histone-lysine methyltransferase family.,similarity:Contains 1 pre-SET domain.,similarity:Contains 1 SET domain.,similarity:Contains 8 ANK repeats.,subcellular location:Associates with euchromatic regions.,subunit:Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2, MBLR, L3MBTL2 and YAF2. Interacts with WIZ and EHMT2.,tissue specificity:Widely expressed., |
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