Name: U-Blot® eIF2α (Phospho Ser51) Rabbit mAb
SKU: WR4667
Price: 288 USD
Availability: InStock

U-Blot® eIF2α (Phospho Ser51) Rabbit mAb

Western Blot analysis using Hela was treated with Thapsigargin(300nM) for 30 minutes lysates,primary antibody was diluted at 1:1000, 4°over night, Dylight 800 secondary antibody(Immunoway:RS23920)was diluted at 1:10000, 37° 1hour.

WR4667 Western Blot analysis using Hela was treated with Thapsigargin(300nM) for 30 minutes lysates,primary antibody was diluted at 1:1000, 4°over night, Dylight 800 secondary antibody(Immunoway:RS23920)was diluted at 1:10000, 37° 1hour.

Price range: $288.00 through $368.00

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50μl

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SKU: WR4667-50

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Product details

Background:The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010],

Specifications

Target	eIF2α
Reactivity	Human,Mouse,Rat
Applications	WB,IHC,IF,ELISA
MW(Calculated)	36kD
MW(Observed)	36kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Phospho
Modification Site	Ser51
Recommended Dilution Ratio	IHC 1:100-1:1000;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000;
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Source
Purification	Protein A
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Clonality	Monoclonal
Clone Number	PT0904R
Specificity	eIF2α (Phospho Ser51) Monoclonal Antibody detects endogenous levels of eIF2α protein only when phosphorylated at S51.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):ELsRR
Gene Name	EIF2S1
Protein Name	Eukaryotic translation initiation factor 2 subunit 1
Other Name	EIF2S1;EIF2A;Eukaryotic translation initiation factor 2 subunit 1;Eukaryotic translation initiation factor 2 subunit alpha;eIF-2-alpha;eIF-2A;eIF-2alpha
Organism-1	Human
Gene ID-1	1965
SwissProt-1	P05198
Organism-2	Mouse
Gene ID-2	13665
SwissProt-2	Q6ZWX6
Organism-3	Rat
Gene ID-3	54318
SwissProt-3	P68101
Organism-4
GeneID-4
SwissProt-4
Cellular Localization	Cytoplasm, Stress granule . Colocalizes with NANOS3 in the stress granules. .
Unnamed: 33