U-Blot® eIF4B Rabbit mAb
WR4312 Western blot analysis of lysates from Jurkat cells, using Sumo 1 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.
Price range: $268.00 through $328.00
Product details
Background:cRequired for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5′-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.,similarity:Contains 1 RRM (RNA recognition motif) domain.,subunit:Self-associates and interacts with EIF3 p170 subunit.
Specifications
| Size | 50μL/100μL |
| Target | eIF4B |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 69kD |
| MW(Observed) | 80kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:2000-1:10000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0748R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | EIF4B |
| Protein Name | Eif4b |
| Other Name | Eukaryotic translation initiation factor 4B; / eIF-4B |
| Species | Human |
| Gene ID | 7341 |
| Uniprot | P23588, |
| Species.1 | Mouse |
| Gene ID.1 | 75705 |
| Uniprot.1 | Q8BGD9, |
| Species.2 | Rat |
| Gene ID.2 | 301442 |
| Uniprot.2 | Q5I0H3 |
| Cellular Localization | cytosol,polysome,eukaryotic translation initiation factor 4F complex,dendrite,neuronal cell body, |
| Tissue Expression | Brain,Colon adenocarcinoma,Epithelium,Placenta, |
| Signaling_pathway | Genetic Information Processing >> Translation >> Nucleocytoplasmic transport |
| Research Areas | >>Nucleocytoplasmic transport; / >>Fluid shear stress and atherosclerosis |
| Function | Function:Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.,similarity:Contains 1 RRM (RNA recognition motif) domain.,subunit:Self-associates and interacts with EIF3 p170 subunit., |
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