Name: Elk1 Monoclonal Antibody
SKU: WM0155
Price: 268 USD
Availability: InStock

Product details

Background:
This gene is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum response element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. This gene produces multiple isoforms by using alternative translational start codons and by alternative splicing. Related pseudogenes have been identified on chromosomes 7 and 14. [provided by RefSeq, Mar 2012],

Specifications

Size	50μL/100μL
Target	Elk-1
Reactivity	Human
Applications	WB, IHC, IF, ELISA
MW(Calculated)	45kD
MW(Observed)
Host Species	Mouse
Isotype
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	WB 1:500-1:2000; IHC 1:200-1:1000; ELISA 1:10000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification	Affinity purification
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	13B3
Immunogen	Purified recombinant fragment of Elk1 expressed in E. Coli.
Specificity	Elk1 Monoclonal Antibody detects endogenous levels of Elk1 protein.
Gene Name	ELK1
Protein Name	ETS domain-containing protein Elk-1
Other Name	ELK1; / ETS domain-containing protein Elk-1
Species	Human
Gene ID	2002
Uniprot	P19419
Species.1
Gene ID.1
Uniprot.1
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Nucleus.
Tissue Expression	Lung and testis.
Signaling_pathway	Cellular Processes >> Cellular community - eukaryotes >> Focal adhesion;Organismal Systems >> Endocrine system >> Insulin signaling pathway;Organismal Systems >> Endocrine system >> GnRH signaling pathway;Organismal Systems >> Endocrine system >> Oxytocin signaling pathway;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: specific types >> Hepatocellular carcinoma;Human Diseases >> Cancer: specific types >> Endometrial cancer;Environmental Information Processing >> Signal transduction >> MAPK signaling pathway;Environmental Information Processing >> Signal transduction >> ErbB signaling pathway;Environmental Information Processing >> Signal transduction >> Ras signaling pathway
Research Areas	>>MAPK signaling pathway; / >>ErbB signaling pathway; / >>Ras signaling pathway; / >>Focal adhesion; / >>Insulin signaling pathway; / >>GnRH signaling pathway; / >>Oxytocin signaling pathway; / >>Leishmaniasis; / >>Hepatitis B; / >>Human cytomegalovirus infection; / >>Human T-cell leukemia virus 1 infection; / >>Pathways in cancer; / >>Proteoglycans in cancer; / >>Endometrial cancer; / >>Hepatocellular carcinoma
Function	Function:Stimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element.,PTM:On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity.,PTM:Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention.,similarity:Belongs to the ETS family.,similarity:Contains 1 ETS DNA-binding domain.,subunit:Interacts in its sumoylated form with PIAS2/PIASX which enhances its transcriptional activator activity.,tissue specificity:Lung and testis.,