Price range: $268.00 through $328.00
Background:
ETS2 is a transcription factor and protooncogene involved in development, apoptosis, and the regulation of telomerase. The protein encoded by this gene binds to the ETS2 promoter and is a strong repressor of ETS2 transcription. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Aug 2015],
| Size | 50μL/100μL |
| Target | ERF |
| Reactivity | Human, Mouse |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 59kD |
| MW(Observed) | |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ERF. AA range:492-541 |
| Specificity | ERF Polyclonal Antibody detects endogenous levels of ERF protein. |
| Gene Name | ERF |
| Protein Name | ETS domain-containing transcription factor ERF |
| Other Name | ERF; / ETS domain-containing transcription factor ERF; / Ets2 repressor factor; / PE-2 |
| Species | Human |
| Gene ID | 2077 |
| Uniprot | P50548, |
| Species.1 | Mouse |
| Gene ID.1 | 13875 |
| Uniprot.1 | P70459 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nucleus. |
| Tissue Expression | Highest levels in testis, ovary, pancreas, and heart. |
| Signaling_pathway | |
| Research Areas | |
| Function | Function:Potent transcriptional repressor that binds to the H1 element of the Ets2 promoter. May regulate other genes involved in cellular proliferation. Required for extraembryonic ectoderm differentiation, ectoplacental cone cavity closure, and chorioallantoic attachment (By similarity). May be important for regulating trophoblast stem cell differentiation.,PTM:Phosphorylated by multiple kinases including probably ERK2. Phosphorylation regulates the activity of ERF.,sequence Caution:The sequence differs from that shown because it seems to be derived from a pre-mRNA.,similarity:Belongs to the ETS family.,similarity:Contains 1 ETS DNA-binding domain.,tissue specificity:Highest levels in testis, ovary, pancreas, and heart., |
