Price range: $268.00 through $328.00
Background:
function:Involved in translation termination in response to the termination codons UAA, UAG and UGA. Stimulates the activity of ERF1. Involved in regulation of mammalian cell growth., similarity:Belongs to the GTP-binding elongation factor family. ERF3 subfamily., subunit:Component of the mRNA surveillance SURF complex, at least composed of ERF1, ERF3 (ERF3A or ERF3B), EEF2, UPF1/RENT1, SMG1, SMG8 and SMG9.,
| Size | 50μL/100μL |
| Target | eRF3a |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 55kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:40000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human GSPT1. AA range:101-150 |
| Specificity | eRF3a Polyclonal Antibody detects endogenous levels of eRF3a protein. |
| Gene Name | GSPT1 |
| Protein Name | Eukaryotic peptide chain release factor GTP-binding subunit ERF3A |
| Other Name | GSPT1; / ERF3A; / Eukaryotic peptide chain release factor GTP-binding subunit ERF3A; / Eukaryotic peptide chain release factor subunit 3a; / eRF3a; / G1 to S phase transition protein 1 homolog |
| Species | Human |
| Gene ID | 2935 |
| Uniprot | P15170, |
| Species.1 | Mouse |
| Gene ID.1 | 14852 |
| Uniprot.1 | Q8R050 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | intracellular,cytosol,translation release factor complex, |
| Tissue Expression | Lung,Testis, |
| Signaling_pathway | |
| Research Areas | >>mRNA surveillance pathway |
| Function | Function:Involved in translation termination in response to the termination codons UAA, UAG and UGA. Stimulates the activity of ERF1. Involved in regulation of mammalian cell growth.,similarity:Belongs to the GTP-binding elongation factor family. ERF3 subfamily.,subunit:Component of the mRNA surveillance SURF complex, at least composed of ERF1, ERF3 (ERF3A or ERF3B), EEF2, UPF1/RENT1, SMG1, SMG8 and SMG9., |
