ERK 8 Polyclonal Antibody
Ucallm_WP12249-50
Price range: $268.00 through $328.00
Product details
Background:
catalytic activity:ATP + a protein = ADP + a phosphoprotein., domain:The N-terminal region (1-20) is the minimal region necessary for ubiquitination and further proteosomal degradation., domain:The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases., enzyme regulation:Activated by threonine and tyrosine phosphorylation. Inhibited by dual specificity phosphatases, such as DUSP1., function:In vitro, phosphorylates MBP., PTM:Dually phosphorylated on Thr-175 and Tyr-177, which activates the enzyme. Autophosphorylated on threonine and tyrosine residues in vitro., PTM:Ubiquitinated. Ubiquitination may allow its tight kinase activity regulation and rapid turnover. May be ubiquitinated by a SCF E3 ligase., similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily., similarity:Contains 1 protein kinase domain., subunit:Interacts with CSK/c-Src, ABL1, RET and TGFB1I1., tissue specificity:Widely expressed with a maximal expression in lung and kidney.,
Specifications
| Size | 50μL/100μL |
| Target | ERK8 |
| Reactivity | Human, Mouse |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 60kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MAPK15. AA range:141-190 |
| Specificity | ERK 8 Polyclonal Antibody detects endogenous levels of ERK 8 protein. |
| Gene Name | MAPK15 |
| Protein Name | Mitogen-activated protein kinase 15 |
| Other Name | MAPK15; / ERK7; / ERK8; / Mitogen-activated protein kinase 15; / MAP kinase 15; / MAPK 15; / Extracellular signal-regulated kinase 7; / ERK-7; / Extracellular signal-regulated kinase 8; / ERK-8 |
| Species | Human |
| Gene ID | 225689 |
| Uniprot | Q8TD08, |
| Species.1 | Mouse |
| Gene ID.1 | 332110 |
| Uniprot.1 | Q80Y86 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasm, cytoskeleton, cilium basal body . Cell junction, tight junction . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole . Cytoplasmic vesicle, autophagosome . Golgi apparatus . Nucleus . Cytoplasm . Cytoplasm, cytoskeleton, spindle . Co-localizes to the cytoplasm only in presence of ESRRA (PubMed:21190936). Translocates to the nucleus upon activation (PubMed:20638370). At prometaphase I, metaphase I (MI), anaphase I, telophase I, and metaphase II (MII) stages, is stably detected at the spindle (By similarity). . |
| Tissue Expression | Widely expressed with a maximal expression in lung and kidney. |
| Signaling_pathway | Organismal Systems >> Immune system >> IL-17 signaling pathway |
| Research Areas | >>IL-17 signaling pathway |
| Function | Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,Domain:The N-terminal region (1-20) is the minimal region necessary for ubiquitination and further proteosomal degradation.,Domain:The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.,enzyme regulation:Activated by threonine and tyrosine phosphorylation. Inhibited by dual specificity phosphatases, such as DUSP1.,Function:In vitro, phosphorylates MBP.,PTM:Dually phosphorylated on Thr-175 and Tyr-177, which activates the enzyme. Autophosphorylated on threonine and tyrosine residues in vitro.,PTM:Ubiquitinated. Ubiquitination may allow its tight kinase activity regulation and rapid turnover. May be ubiquitinated by a SCF E3 ligase.,similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with CSK/c-Src, ABL1, RET and TGFB1I1.,tissue specificity:Widely expressed with a maximal expression in lung and kidney., |
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