Price range: $268.00 through $328.00
Background:
FOXH1 encodes a human homolog of Xenopus forkhead activin signal transducer-1. FOXH1 protein binds SMAD2 and activates an activin response element via binding the DNA motif TGT(G/T)(T/G)ATT. [provided by RefSeq, Jul 2008],
| Size | 50μL/100μL |
| Target | FAST-1/2 |
| Reactivity | Human, Mouse |
| Applications | WB, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 40kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IF 1:200-1:1000; ELISA 1:10000; Not yet tested in other applications. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human FOXH1. AA range:10-59 |
| Specificity | FAST-1/2 Polyclonal Antibody detects endogenous levels of FAST-1/2 protein. |
| Gene Name | FOXH1 |
| Protein Name | Forkhead box protein H1 |
| Other Name | FOXH1; / FAST1; / FAST2; / Forkhead box protein H1; / Forkhead activin signal transducer 1; / Fast-1; / hFAST-1; / Forkhead activin signal transducer 2; / Fast-2 |
| Species | Human |
| Gene ID | 8928 |
| Uniprot | O75593, |
| Species.1 | Mouse |
| Gene ID.1 | 14106 |
| Uniprot.1 | O88621 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nucleus. |
| Tissue Expression | Ubiquitous. |
| Signaling_pathway | |
| Research Areas | |
| Function | Domain:The FM region is required for binding smad2/smad4 complexes. FM2 is more effective than FM1 and only interacts with phosphorylated smad2 that is in an activated smad complex.,Function:Transcriptional activator. Recognizes and binds to the DNA sequence 5'-TGT[GT][GT]ATT-3'. Required for induction of the goosecoid (GSC) promoter by TGF-beta or activin signaling. Forms a transcriptionally active complex containing FOXH1/SMAD2/SMAD4 on a site on the GSC promoter called TARE (TGF-beta/activin response element).,similarity:Contains 1 fork-head DNA-binding domain.,subunit:Interacts with the MH2 domains of SMAD2 and SMAD3.,tissue specificity:Ubiquitous., |
