SKU: WP3267 Categories: ,

FAT2 Polyclonal Antibody

Price range: $268.00 through $328.00

SKU: WP3267-100
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Product details

Background:

This gene is the second identified human homolog of the Drosophila fat gene, which encodes a tumor suppressor essential for controlling cell proliferation during Drosophila development. The gene product is a member of the cadherin superfamily, a group of integral membrane proteins characterized by the presence of cadherin-type repeats. In addition to containing 34 tandem cadherin-type repeats, the gene product has two epidermal growth factor (EGF)-like repeats and one laminin G domain. This protein most likely functions as a cell adhesion molecule, controlling cell proliferation and playing an important role in cerebellum development. [provided by RefSeq, Jul 2008],

Specifications

Size50μL/100μL
TargetFAT2
ReactivityHuman, Mouse, Rat
ApplicationsIHC, IF
MW(Calculated)
MW(Observed)478kD
Host SpeciesRabbit
IsotypeIgG
Conjugate/ModificationUnmodified
Recommended Dilution RatioIHC 1:50-300; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationLiquid in PBS containing 50% glycerol,0.5% BSA and 0.02% sodium azide.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenSynthesized peptide derived from part region of human protein
SpecificityFAT2 Polyclonal Antibody detects endogenous levels of protein.
Gene NameFAT2 CDHF8 KIAA0811 MEGF1
Protein NameProtocadherin Fat 2 (hFat2) (Cadherin family member 8) (Multiple epidermal growth factor-like domains protein 1) (Multiple EGF-like domains protein 1)
Other Name
SpeciesHuman
Gene ID2196
UniprotQ9NYQ8,
Species.1Mouse
Gene ID.1
Uniprot.1Q5F226,
Species.2Rat
Gene ID.2
Uniprot.2O88277
Cellular LocalizationCell membrane; Single-pass type I membrane protein . Cell junction . Golgi apparatus, trans-Golgi network . Localized at adhesion zippers (early state of adherens junctions) of keratinocytes. .
Tissue ExpressionExpressed in epidermal keratinocytes, infant brain, cerebellum, and also in a variety of tumors, such as pancreatic cancer, diffuse type gastric cancer, ovarian cancer, esophageal cancer, skin squamous cell carcinoma, head and neck cancer. Not expressed in melanoma cell line A375 cells, normal epidermal melanocytes or normal dermal fibroblasts. Expressed in epidermal keratinocytes and squamous cell carcinoma (at protein level).
Signaling_pathway
Research Areas
Functionsimilarity:Contains 1 laminin G-like domain.,similarity:Contains 2 EGF-like domains.,similarity:Contains 32 cadherin domains.,
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