Fnk Polyclonal Antibody
Ucallm_WP12325-50
Price range: $268.00 through $328.00
Product details
Background:
The protein encoded by this gene is a member of the highly conserved polo-like kinase family of serine/threonine kinases. Members of this family are characterized by an amino-terminal kinase domain and a carboxy-terminal bipartite polo box domain that functions as a substrate-binding motif and a cellular localization signal. Polo-like kinases are important regulators of cell cycle progression. This gene has also been implicated in stress responses and double-strand break repair. In human cell lines, this protein is reported to associate with centrosomes in a microtubule-dependent manner, and during mitosis, the protein becomes localized to the mitotic apparatus. Expression of a kinase-defective mutant results in abnormal cell morphology caused by changes in microtubule dynamics and mitotic arrest followed by apoptosis. [provided by RefSeq, Sep 2015],
Specifications
| Size | 50μL/100μL |
| Target | Fnk |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, ELISA |
| MW(Calculated) | |
| MW(Observed) | 70kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; ELISA 1:10000; Not yet tested in other applications. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PLK3. AA range:231-280 |
| Specificity | Fnk Polyclonal Antibody detects endogenous levels of Fnk protein. |
| Gene Name | PLK3 |
| Protein Name | Serine/threonine-protein kinase PLK3 |
| Other Name | PLK3; / CNK; / FNK; / PRK; / Serine/threonine-protein kinase PLK3; / Cytokine-inducible serine/threonine-protein kinase; / FGF-inducible kinase; / Polo-like kinase 3; / PLK-3; / Proliferation-related kinase |
| Species | Human |
| Gene ID | 1263 |
| Uniprot | Q9H4B4, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q60806, |
| Species.2 | Rat |
| Gene ID.2 | 58936 |
| Uniprot.2 | Q9R011 |
| Cellular Localization | Cytoplasm. Nucleus. Nucleus, nucleolus. Golgi apparatus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Translocates to the nucleus upon cisplatin treatment. Localizes to the Golgi apparatus during interphase. According to a report, PLK3 localizes only in the nucleolus and not in the centrosome, or in any other location in the cytoplasm (PubMed:17264206). The discrepancies in results may be explained by the PLK3 antibody specificity, by cell line-specific expression or post-translational modifications. . |
| Tissue Expression | Transcripts are highly detected in placenta, lung, followed by skeletal muscle, heart, pancreas, ovaries and kidney and weakly detected in liver and brain. May have a short half-live. In cells of hematopoietic origin, strongly and exclusively detected in terminally differentiated macrophages. Transcript expression appears to be down-regulated in primary lung tumor. |
| Signaling_pathway | Environmental Information Processing >> Signal transduction >> FoxO signaling pathway |
| Research Areas | >>FoxO signaling pathway; / >>C-type lectin receptor signaling pathway; / >>Tuberculosis |
| Function | Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,Function:Serine/threonine protein kinase involved in regulating M phase functions during the cell cycle. May also be part of the signaling network controlling cellular adhesion. In vitro, is able to phosphorylate CDC25C and casein.,induction:Cytokine and cellular adhesion trigger FNK induction.,PTM:Phosphorylated as cells enter mitosis and dephosphorylated as cells exit mitosis.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 2 POLO box domains.,subunit:Binds to the calcium/integrin-binding protein (CIB). This interaction probably occurs via the POLO-box domain.,tissue specificity:Transcripts are highly detected in placenta, lung, followed by skeletal muscle, heart, pancreas, ovaries and kidney and weakly detected in liver and brain. May have a short half-live. In cells of hematopoietic origin, strongly and exclusively detected in terminally differentiated macrophages. Transcript expression appears to be down-regulated in primary lung tumor., |
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