Price range: $268.00 through $328.00
Background:
The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008],
| Size | 50μL/100μL |
| Target | FOXP3 |
| Reactivity | Human, Mouse |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 47kD |
| MW(Observed) | |
| Host Species | Mouse |
| Isotype | |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:200-1:1000; IF 1:200-1:1000; ELISA 1:10000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | Affinity purification |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | 4D12 |
| Immunogen | Purified recombinant fragment of human FoxP3 expressed in E. Coli. |
| Specificity | FoxP3 Monoclonal Antibody detects endogenous levels of FoxP3 protein. |
| Gene Name | FOXP3 |
| Protein Name | Forkhead box protein P3 |
| Other Name | FOXP3; / IPEX; / JM2; / Forkhead box protein P3; / Scurfin |
| Species | Human |
| Gene ID | 50943 |
| Uniprot | Q9BZS1, |
| Species.1 | Mouse |
| Gene ID.1 | 20371 |
| Uniprot.1 | Q99JB6 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nucleus . Cytoplasm . Predominantly expressed in the cytoplasm in activated conventional T-cells whereas predominantly expressed in the nucleus in regulatory T-cells (Treg). The 41 kDa form derived by proteolytic processing is found exclusively in the chromatin fraction of activated Treg cells (By similarity). . |
| Tissue Expression | |
| Signaling_pathway | Organismal Systems >> Immune system >> Th17 cell differentiation;Human Diseases >> Immune disease >> Inflammatory bowel disease |
| Research Areas | >>Th17 cell differentiation; / >>Inflammatory bowel disease |
| Function | Disease:Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy.,Function:Probable transcription factor. Plays a critical role in the control of immune response.,online information:FOXP3 entry,online information:FOXP3 mutation db,similarity:Contains 1 C2H2-type zinc finger.,similarity:Contains 1 fork-head DNA-binding domain., |
