Price range: $268.00 through $328.00
Background:
frizzled class receptor 5(FZD5) Homo sapiens Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD5 protein is believed to be the receptor for the Wnt5A ligand. [provided by RefSeq, Jul 2008],
| Size | 50μL/100μL |
| Target | Frizzled-5/8 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 75kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IF 1:200-1:1000; ELISA 1:20000; Not yet tested in other applications. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human FZD8. AA range:31-80 |
| Specificity | Frizzled-5/8 Polyclonal Antibody detects endogenous levels of Frizzled-5/8 protein. |
| Gene Name | FZD5/FZD8 |
| Protein Name | Frizzled-5/8 |
| Other Name | FZD5; / C2orf31; / Frizzled-5; / Fz-5; / hFz5; / FzE5; / FZD8; / Frizzled-8; / Fz-8; / hFz8 |
| Species | Human |
| Gene ID | 8325;7855 |
| Uniprot | Q13467;Q9H461, |
| Species.1 | Mouse |
| Gene ID.1 | 14367;14370, |
| Uniprot.1 | |
| Species.2 | Rat |
| Gene ID.2 | 317674;100909849 |
| Uniprot.2 | Q8CHL0;Q498S8 |
| Cellular Localization | Cell membrane; Multi-pass membrane protein . Golgi apparatus membrane; Multi-pass membrane protein . Cell junction, synapse . Perikaryon . Cell projection, dendrite . Cell projection, axon . Localized at the plasma membrane and also found at the Golgi. . |
| Tissue Expression | Oesophageal carcinoma,Retina, |
| Signaling_pathway | Cellular Processes >> Cellular community - eukaryotes >> Signaling pathways regulating pluripotency of stem cells;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: specific types >> Hepatocellular carcinoma;Human Diseases >> Cancer: specific types >> Gastric cancer;Human Diseases >> Cancer: specific types >> Basal cell carcinoma;Human Diseases >> Cancer: specific types >> Breast cancer;Human Diseases >> Neurodegenerative disease >> Alzheimer disease;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases;Environmental Information Processing >> Signal transduction >> Wnt signaling pathway;Environmental Information Processing >> Signal transduction >> Hippo signaling pathway;Environmental Information Processing >> Signal transduction >> mTOR signaling pathway |
| Research Areas | >>mTOR signaling pathway; / >>Wnt signaling pathway; / >>Hippo signaling pathway; / >>Signaling pathways regulating pluripotency of stem cells; / >>Melanogenesis; / >>Cushing syndrome; / >>Alzheimer disease; / >>Pathways of neurodegeneration - multiple diseases; / >>Human papillomavirus infection; / >>Pathways in cancer; / >>Proteoglycans in cancer; / >>Basal cell carcinoma; / >>Breast cancer; / >>Hepatocellular carcinoma; / >>Gastric cancer |
| Function | Domain:Lys-Thr-X-X-X-Trp motif is involved in the activation of the Wnt/beta-catenin signaling pathway.,Domain:The FZ domain is involved in binding with Wnt ligands.,Domain:The PDZ-binding motif mediates interaction with GOPC.,Function:Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Interacts specifically with Wnt5A to induce the beta-catenin pathway.,similarity:Belongs to the G-protein coupled receptor Fz/Smo family.,similarity:Contains 1 FZ (frizzled) domain.,subcellular location:Localized at the plasma membrane and also found at the Golgi.,subunit:Interacts with GOPC., |
