U-Blot® FRS2 Rabbit mAb
WR4689 Western Blot analysis using SH-SY5Y lysates,primary antibody was diluted at 1:1000, 4°over night, Dylight 800 secondary antibody(WS0446)was diluted at 1:10000, 37° 1hour.
Price range: $268.00 through $328.00
Product details
Background:function:Adapter protein that links FGR and NGF receptors to downstream signaling pathways. Involved in the activation of MAP kinases. Modulates signaling via SHC1 by competing for a common binding site on NTRK1.,PTM:Phosphorylated on tyrosine residues upon stimulation by NGF.,PTM:Ubiquitinated when tyrosine phosphorylated and in a complex with GRB2. The unphosphorylated form is not subject to ubiquitination.,sequence caution:Translated as stop.,similarity:Contains 1 IRS-type PTB domain.,subcellular location:Cytoplasmic, membrane-bound.,subunit:Part of a complex containing FRS2, GRB2 and SOS1. Part of a complex containing GRB2 and CBL. Binds RET (By similarity). Binds FGFR1, SUC1, NTRK1, NTRK2, NTRK3 and SRC. The tyrosine-phosphorylated protein binds the SH2 domains of GRB2 and PTPN11.,tissue specificity:Highly expressed in heart, brain, spleen, lung, liver, skeletal muscle, kidney and testis.,
Specifications
| Size | 50μL/100μL |
| Target | FRS2 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IF, IP, ELISA |
| MW(Calculated) | 57kD |
| MW(Observed) | 80kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1008R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | FRS2 |
| Protein Name | Fibroblast growth factor receptor substrate 2 |
| Other Name | FRS2; / Fibroblast growth factor receptor substrate 2; / FGFR substrate 2; / FGFR-signaling adaptor SNT; / Suc1-associated neurotrophic factor target 1; / SNT-1 |
| Species | Human |
| Gene ID | 10818 |
| Uniprot | Q8WU20, |
| Species.1 | Mouse |
| Gene ID.1 | 327826 |
| Uniprot.1 | Q8C180 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Endomembrane system. Cytoplasmic, membrane-bound. |
| Tissue Expression | Highly expressed in heart, brain, spleen, lung, liver, skeletal muscle, kidney and testis. |
| Signaling_pathway | Organismal Systems >> Nervous system >> Neurotrophin signaling pathway |
| Research Areas | >>Thermogenesis; / >>Neurotrophin signaling pathway; / >>Proteoglycans in cancer |
| Function | Function:Adapter protein that links FGR and NGF receptors to downstream signaling pathways. Involved in the activation of MAP kinases. Modulates signaling via SHC1 by competing for a common binding site on NTRK1.,PTM:Phosphorylated on tyrosine residues upon stimulation by NGF.,PTM:Ubiquitinated when tyrosine phosphorylated and in a complex with GRB2. The unphosphorylated form is not subject to ubiquitination.,sequence Caution:Translated as stop.,similarity:Contains 1 IRS-type PTB domain.,subcellular location:Cytoplasmic, membrane-bound.,subunit:Part of a complex containing FRS2, GRB2 and SOS1. Part of a complex containing GRB2 and CBL. Binds RET (By similarity). Binds FGFR1, SUC1, NTRK1, NTRK2, NTRK3 and SRC. The tyrosine-phosphorylated protein binds the SH2 domains of GRB2 and PTPN11.,tissue specificity:Highly expressed in heart, brain, spleen, lung, liver, skeletal muscle, kidney and testis., |
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