SKU: WP12386 Categories: ,

G2A Polyclonal Antibody

Price range: $268.00 through $328.00

SKU: WP12386-100
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Product details

Background:

This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor (GPCR) superfamily. The receptors are seven-pass transmembrane proteins that respond to extracellular cues and activate intracellular signal transduction pathways. This protein was reported to be a receptor for lysophosphatidylcholine action, but PubMedID: 15653487 retracts this finding and instead suggests this protein to be an effector of lysophosphatidylcholine action. This protein may have proton-sensing activity and may be a receptor for oxidized free fatty acids. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013],

Specifications

Size50μL/100μL
TargetGPR132
ReactivityHuman, Mouse, Monkey
ApplicationsWB, IF, ELISA
MW(Calculated)
MW(Observed)42kD
Host SpeciesRabbit
IsotypeIgG
Conjugate/ModificationUnmodified
Recommended Dilution RatioWB 1:500-1:2000; ELISA 1:20000; IF 1:100-300; Not yet tested in other applications.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenThe antiserum was produced against synthesized peptide derived from human GPR132. AA range:293-342
SpecificityG2A Polyclonal Antibody detects endogenous levels of G2A protein.
Gene NameGPR132
Protein NameProbable G-protein coupled receptor 132
Other NameGPR132; / G2A; / Probable G-protein coupled receptor 132; / G2 accumulation protein
SpeciesHuman
Gene ID29933
UniprotQ9UNW8,
Species.1Mouse
Gene ID.156696
Uniprot.1Q9Z282
Species.2
Gene ID.2
Uniprot.2
Cellular LocalizationCell membrane; Multi-pass membrane protein . Internalized and accumulated in endosomal compartments. LPC triggers the relocalization from the endosomal compartment to the cell surface (By similarity). .
Tissue ExpressionHighly expressed in macrophages and hematopoietic tissues rich in lymphocytes, like spleen and thymus. Weakly expressed in heart and lung. In atherosclerotic plaques, expression is observed around the lipid core and at the shoulder region.
Signaling_pathway
Research Areas
FunctionCaution:Was originally (PubMed:11474113) thought to be a receptor for lysophosphatidylcholine (LPC) and sphingosylphosphorylcholine (SPC), However, this work has been retracted.,Function:Receptor for an unknown ligand. Activates a G alpha protein, most likely G alpha(q). May be involved in apoptosis. Functions at the G2/M checkpoint to delay mitosis. May serve as a mechanism for T- and B-cells, and other cell types, to slow their proliferation and repair damaged DNA to ensure proper replication.,induction:By stress and DNA-damaging agents.,similarity:Belongs to the G-protein coupled receptor 1 family.,subcellular location:Internalized and accumulated in endosomal compartments. LPC triggers the relocalization from the endosomal compartment to the cell surface.,tissue specificity:Highly expressed in macrophages and hematopoietic tissues rich in lymphocytes, like spleen and thymus. Weakly expressed in heart and lung. In atherosclerotic plaques, expression is observed around the lipid core and at the shoulder region.,
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