Name: GABA B Receptor 2 Polyclonal Antibody
SKU: WP5865
Price: 268 USD
Availability: InStock

Product details

Background:

The multi-pass membrane protein encoded by this gene belongs to the G-protein coupled receptor 3 family and GABA-B receptor subfamily. The GABA-B receptors inhibit neuronal activity through G protein-coupled second-messenger systems, which regulate the release of neurotransmitters, and the activity of ion channels and adenylyl cyclase. This receptor subunit forms an active heterodimeric complex with GABA-B receptor subunit 1, neither of which is effective on its own. Allelic variants of this gene have been associated with nicotine dependence.[provided by RefSeq, Jan 2010],

Specifications

Size	50μL/100μL
Target	GABBR2
Reactivity	Human, Mouse, Rat
Applications	IHC, IF
MW(Calculated)
MW(Observed)	106kD
Host Species	Rabbit
Isotype	IgG
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:100-200; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration	1 mg/ml
Clonality	Polyclonal
Clone Number
Immunogen	Synthetic Peptide of GABA B Receptor 2 AA range: 785-835
Specificity	GABA B Receptor 2 protein(A228) detects endogenous levels of GABA B Receptor 2
Gene Name	GABBR2
Protein Name	Gamma-aminobutyric acid type B receptor subunit 2 (GABA-B receptor 2) (GABA-B-R2) (GABA-BR2) (GABABR2) (Gb2) (G-protein coupled receptor 51) (HG20)
Other Name	GABBR2; / GPR51; / GPRC3B; / Gamma-aminobutyric acid type B receptor subunit 2; / GABA-B receptor 2; / GABA-B-R2; / GABA-BR2; / GABABR2; / Gb2; / G-protein coupled receptor 51; / HG20
Species	Human
Gene ID	9568
Uniprot	O75899,
Species.1	Mouse
Gene ID.1
Uniprot.1	Q80T41,
Species.2	Rat
Gene ID.2
Uniprot.2	O88871
Cellular Localization	Cell membrane; Multi-pass membrane protein . Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein . Coexpression of GABBR1 and GABBR2 is required for GABBR1 maturation and transport to the plasma membrane. In contrast, GABBR2 does not depend on GABBR1 for transport to the cell membrane. .
Tissue Expression	Highly expressed in brain, especially in cerebral cortex, thalamus, hippocampus, frontal, occipital and temporal lobe, occipital pole and cerebellum, followed by corpus callosum, caudate nucleus, spinal cord, amygdala and medulla (PubMed:10087195, PubMed:10328880, PubMed:10727622, PubMed:9872744). Weakly expressed in heart, testis and skeletal muscle (PubMed:10087195, PubMed:10727622).
Signaling_pathway	Organismal Systems >> Endocrine system >> Estrogen signaling pathway;Environmental Information Processing >> Signal transduction >> cAMP signaling pathway
Research Areas	>>cAMP signaling pathway; / >>Neuroactive ligand-receptor interaction; / >>GABAergic synapse; / >>Taste transduction; / >>Estrogen signaling pathway; / >>GnRH secretion; / >>Morphine addiction
Function	Domain:Alpha-helical parts of the C-terminal intracellular region mediate heterodimeric interaction with GABA-B receptor 1.,Function:Receptor for GABA. The activity of this receptor is mediated by G-proteins that inhibit adenylyl cyclase activity, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipids hydrolysis. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA-B-R inhibit neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA-B-R decrease neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception.,similarity:Belongs to the G-protein coupled receptor 3 family. GABA-B receptor subfamily.,subcellular location:Moreover coexpression of GABA-B-R1 and GABA-B-R2 appears to be a prerequisite for maturation and transport of GABA-B-R1 to the plasma membrane.,subunit:Heterodimer of GABA-B-R1 and GABA-B-R2. Neither of which is effective on its own and homodimeric assembly does not seem to happen. Interacts with ATF4 via its C-terminal region.,tissue specificity:Highly expressed in brain, especially in cerebral cortex, thalamus, hippocampus, frontal, occipital and temporal lobe, occipital pole and cerebellum, followed by corpus callosum, caudate nucleus, spinal cord, amygdala and medulla. Weakly expressed in heart, testis and skeletal muscle.,