GAK Polyclonal Antibody
Ucallm_WP12406-50
Price range: $268.00 through $328.00
Product details
Background:
cyclin G associated kinase(GAK) Homo sapiens In all eukaryotes, the cell cycle is governed by cyclin-dependent protein kinases (CDKs), whose activities are regulated by cyclins and CDK inhibitors in a diverse array of mechanisms that involve the control of phosphorylation and dephosphorylation of Ser, Thr or Tyr residues. Cyclins are molecules that possess a consensus domain called the 'cyclin box.' In mammalian cells, 9 cyclin species have been identified, and they are referred to as cyclins A through I. Cyclin G is a direct transcriptional target of the p53 tumor suppressor gene product and thus functions downstream of p53. GAK is an association partner of cyclin G and CDK5. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015],
Specifications
| Size | 50μL/100μL |
| Target | GAK |
| Reactivity | Human, Mouse |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 144kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:5000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human GAK. AA range:101-150 |
| Specificity | GAK Polyclonal Antibody detects endogenous levels of GAK protein. |
| Gene Name | GAK |
| Protein Name | Cyclin-G-associated kinase |
| Other Name | GAK; / Cyclin-G-associated kinase |
| Species | Human |
| Gene ID | 2580 |
| Uniprot | O14976, |
| Species.1 | Mouse |
| Gene ID.1 | 231580 |
| Uniprot.1 | Q99KY4 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasm, perinuclear region . Golgi apparatus, trans-Golgi network . Cell junction, focal adhesion . Localizes to the perinuclear area and to the trans-Golgi network. Also seen on the plasma membrane, probably at focal adhesions. |
| Tissue Expression | Ubiquitous. Highest in testis. |
| Signaling_pathway | |
| Research Areas | |
| Function | Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,Function:Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.,similarity:Contains 1 C2 tensin-type domain.,similarity:Contains 1 J domain.,similarity:Contains 1 phosphatase tensin-type domain.,similarity:Contains 1 protein kinase domain.,subcellular location:Localizes to the perinuclear area and to the trans-Golgi network. Also seen on the plasma membrane, probably at focals adhesions.,tissue specificity:Ubiquitous. Highest in testis., |
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