Name: GAPDH Polyclonal Antibody
SKU: WP5859
Price: 268 USD
Availability: InStock

Product details

Background:

glyceraldehyde-3-phosphate dehydrogenase(GAPDH) Homo sapiens This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferri

Specifications

Size	50μL/100μL
Target	GAPDH
Reactivity	Zebrafish
Applications	WB
MW(Calculated)
MW(Observed)	37kD
Host Species	Rabbit
Isotype	IgG
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	WB 1:2000-5000
Formulation	PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Polyclonal
Clone Number
Immunogen	Recombinant Protein of Glyceraldehyde-3-phosphate dehydrogenase
Specificity	The antibody detects Zebrafish endogenous GAPDH protein.
Gene Name	GAPDH
Protein Name	Glyceraldehyde-3-phosphate dehydrogenase
Other Name	GAPDH; / GAPD; / CDABP0047; / OK/SW-cl.12; / Glyceraldehyde-3-phosphate dehydrogenase; / GAPDH; / Peptidyl-cysteine S-nitrosylase GAPDH
Species	Human
Gene ID	2597
Uniprot	P04406
Species.1
Gene ID.1
Uniprot.1
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Cytoplasm, cytosol . Nucleus . Cytoplasm, perinuclear region . Membrane . Cytoplasm, cytoskeleton . Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions (PubMed:12829261). .
Tissue Expression	Astrocytoma,Brain,Cajal-Retzius cell,Colon adenocarcinoma,Epitheliu
Signaling_pathway	Human Diseases >> Neurodegenerative disease >> Alzheimer disease;Environmental Information Processing >> Signal transduction >> HIF-1 signaling pathway
Research Areas	>>Glycolysis / Gluconeogenesis; / >>Metabolic pathways; / >>Carbon metabolism; / >>Biosynthesis of amino acids; / >>HIF-1 signaling pathway; / >>Alzheimer disease; / >>Pathogenic Escherichia coli infection; / >>Salmonella infection; / >>Diabetic cardiomyopathy
Function	Catalytic activity:D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH.,Function:Independent of its glycolytic activity it is also involved in membrane trafficking in the early secretory pathway.,online information:Glyceraldehyde 3-phosphate dehydrogenase entry,pathway:Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1.,pathway:Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.,PTM:Reversible S-nitrosylation of Cys-152 inhibits enzymatic activity and increases endogenous ADP-ribosylation, which inhibits the enzyme in a non-reversible manner. The latter modification is more likely to be a pathophysiological event associated with inhibition of gluconeogenesis.,sequence Caution:Differs quite extensively.,similarity:Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.,subcellular location:Postnuclear and Perinuclear regions.,subunit:Homotetramer.,subunit:Homotetramer. Binds PRKCI.,