Name: U-Blot® GAPDH Rabbit mAb
SKU: WR4367
Price: 268 USD
Availability: InStock

U-Blot® GAPDH Rabbit mAb

Western Blot analysis using Hela lysates,primary antibody was diluted at 1:1000, 4°over night, Dylight 800 secondary antibody(WS0446)was diluted at 1:10000, 37° 1hour.

WR4367 Western Blot analysis using Hela lysates,primary antibody was diluted at 1:1000, 4°over night, Dylight 800 secondary antibody(WS0446)was diluted at 1:10000, 37° 1hour.

Price range: $268.00 through $328.00

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100μl

50μl

Clear

SKU: WR4367-50

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Product details

Background:glyceraldehyde-3-phosphate dehydrogenase(GAPDH) Homo sapiens This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferri

Specifications

Size	50μL/100μL
Target	GAPDH
Reactivity	Human, Mouse, Rat, Dog, Monkey, Chicken,
Applications	WB, IHC, IF, ELISA
MW(Calculated)	38kD
MW(Observed)	38kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:2000-1:5000; WB 1:10000-1:50000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM0582R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	GAPDH
Protein Name	Glyceraldehyde-3-phosphate dehydrogenase
Other Name	GAPDH; / GAPD; / CDABP0047; / OK/SW-cl.12; / Glyceraldehyde-3-phosphate dehydrogenase; / GAPDH; / Peptidyl-cysteine S-nitrosylase GAPDH
Species	Human
Gene ID	2597
Uniprot	P04406,
Species.1	Mouse
Gene ID.1	100042025
Uniprot.1	P16858,
Species.2	Rat
Gene ID.2	24383
Uniprot.2	P04797
Cellular Localization	Cytoplasm, Nucleus
Tissue Expression	Astrocytoma,Brain,Cajal-Retzius cell,Colon adenocarcinoma,Epitheliu
Signaling_pathway	Human Diseases >> Neurodegenerative disease >> Alzheimer disease;Environmental Information Processing >> Signal transduction >> HIF-1 signaling pathway
Research Areas	>>Glycolysis / Gluconeogenesis; / >>Metabolic pathways; / >>Carbon metabolism; / >>Biosynthesis of amino acids; / >>HIF-1 signaling pathway; / >>Alzheimer disease; / >>Pathogenic Escherichia coli infection; / >>Salmonella infection; / >>Diabetic cardiomyopathy
Function	Catalytic activity:D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH.,Function:Independent of its glycolytic activity it is also involved in membrane trafficking in the early secretory pathway.,online information:Glyceraldehyde 3-phosphate dehydrogenase entry,pathway:Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1.,pathway:Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.,PTM:Reversible S-nitrosylation of Cys-152 inhibits enzymatic activity and increases endogenous ADP-ribosylation, which inhibits the enzyme in a non-reversible manner. The latter modification is more likely to be a pathophysiological event associated with inhibition of gluconeogenesis.,sequence Caution:Differs quite extensively.,similarity:Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.,subcellular location:Postnuclear and Perinuclear regions.,subunit:Homotetramer.,subunit:Homotetramer. Binds PRKCI.,