U-Blot® GCLC Rabbit mAb
WR4392 Hela cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with primary antibody 1:1000. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.
Price range: $268.00 through $328.00
Product details
Background:Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.[provided by RefSeq, Oct 2010],
Specifications
| Size | 50μL/100μL |
| Target | GCLC |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IF, IP, ELISA |
| MW(Calculated) | 73kD |
| MW(Observed) | 73kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0611R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | GCLC |
| Protein Name | Glutamate--cysteine ligase catalytic subunit |
| Other Name | GCLC; / GLCL; / GLCLC; / Glutamate--cysteine ligase catalytic subunit; / GCS heavy chain; / Gamma-ECS; / Gamma-glutamylcysteine synthetase |
| Species | Human |
| Gene ID | 2729 |
| Uniprot | P48506, |
| Species.1 | Mouse |
| Gene ID.1 | 14629 |
| Uniprot.1 | P97494, |
| Species.2 | Rat |
| Gene ID.2 | 25283 |
| Uniprot.2 | P19468 |
| Cellular Localization | Cytoplasm |
| Tissue Expression | Foreskin fibroblast,Hippocampus,Liver,Testis,Thalamus, |
| Signaling_pathway | Cellular Processes >> Cell growth and death >> Ferroptosis |
| Research Areas | >>Cysteine and methionine metabolism; / >>Glutathione metabolism; / >>Metabolic pathways; / >>Biosynthesis of cofactors; / >>Ferroptosis |
| Function | Catalytic activity:ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.,Disease:Defects in GCLC are the cause of hemolytic anemia [MIM:230450].,enzyme regulation:Feedback inhibition by glutathione.,pathway:Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.,similarity:Belongs to the glutamate--cysteine ligase type 3 family.,subunit:Heterodimer of a catalytic heavy chain and a regulatory light chain., |
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