Price range: $268.00 through $328.00
Background:
This gene encodes the beta subunit of the soluble guanylate cyclase (sGC), which catalyzes the conversion of GTP (guanosine triphosphate) to cGMP (cyclic guanosine monophosphate). The encoded protein contains an HNOX domain, which serves as a receptor for ligands such as nitric oxide, oxygen and nitrovasodilator drugs. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014],
| Size | 50μL/100μL |
| Target | GCS-β-1 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 70kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Guanylate Cyclase beta. AA range:10-59 |
| Specificity | GCS-β-1 Polyclonal Antibody detects endogenous levels of GCS-β-1 protein. |
| Gene Name | GUCY1B3 |
| Protein Name | Guanylate cyclase soluble subunit beta-1 |
| Other Name | GUCY1B3; / GUC1B3; / GUCSB3; / GUCY1B1; / Guanylate cyclase soluble subunit beta-1; / GCS-beta-1; / Guanylate cyclase soluble subunit beta-3; / GCS-beta-3; / Soluble guanylate cyclase small subunit |
| Species | Human |
| Gene ID | 2983 |
| Uniprot | Q02153, |
| Species.1 | Mouse |
| Gene ID.1 | 54195 |
| Uniprot.1 | O54865, |
| Species.2 | Rat |
| Gene ID.2 | 25202 |
| Uniprot.2 | P20595 |
| Cellular Localization | Cytoplasm . |
| Tissue Expression | Detected in brain cortex and cerebellum (at protein level). |
| Signaling_pathway | Cellular Processes >> Cellular community - eukaryotes >> Gap junction;Organismal Systems >> Immune system >> Platelet activation;Organismal Systems >> Endocrine system >> Oxytocin signaling pathway;Organismal Systems >> Circulatory system >> Vascular smooth muscle contraction;Environmental Information Processing >> Signal transduction >> cGMP-PKG signaling pathway |
| Research Areas | >>Purine metabolism; / >>Metabolic pathways; / >>cGMP-PKG signaling pathway; / >>Vascular smooth muscle contraction; / >>Gap junction; / >>Platelet activation; / >>Circadian entrainment; / >>Long-term depression; / >>Oxytocin signaling pathway; / >>Renin secretion; / >>Salivary secretion |
| Function | Catalytic activity:GTP = 3',5'-cyclic GMP + diphosphate.,cofactor:Binds 1 or 2 heme groups per heterodimer.,enzyme regulation:Activated by nitric oxide in the presence of magnesium or manganese ions.,miscellaneous:There are two types of guanylate cyclases: soluble forms and membrane-associated receptor forms.,similarity:Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.,similarity:Contains 1 guanylate cyclase domain.,subunit:Heterodimer of an alpha and a beta chain., |
