Name: GLSK rabbit pAb
SKU: WP4413
Price: 268 USD
Availability: InStock

Product details

Background:

This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012],

Specifications

Target	GLSK
Reactivity	Human, Mouse, Rat
Applications	WB
MW(Calculated)	74kD
MW(Observed)
Host Species	Rabbit
Isotype	IgG
Conjugate/Modification	Unmodified
Modification
Recommended Dilution Ratio	WB 1:500-2000
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration	1 mg/ml
Clonality	Polyclonal
Clone Number
Immunogen	Synthesized peptide derived from human GLSK AA range: 162-212
Sequence
Specificity	This antibody detects endogenous levels of GLSK at Human/Mouse/Rat
Gene Name	GLS GLS1 KIAA0838
Protein Name	GLSK
Other Name
Species	Human
Gene ID-1	2744
Uniprot	O94925,
Species.1	Mouse
Gene ID-2	14660
Uniprot.1	D3Z7P3,
Species.2	Rat
Gene ID-3	24398
Uniprot.2	P13264
Organism-4
Gene ID-4
SwissProt-4
Background	This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012],
Cellular Localization	[Isoform 1]: Mitochondrion . Cytoplasm, cytosol . The 74-kDa cytosolic precursor is translocated into the mitochondria and processed via a 72-kDa intermediate to yield the mature 68- and 65-kDa subunits. .; [Isoform 3]: Mitochondrion .; [Glutaminase kidney isoform, mitochondrial 68 kDa chain]: Mitochondrion matrix . Produced by the proteolytic processing of the 74-kDa cytosolic precursor. .; [Glutaminase kidney isoform, mitochondrial 65 kDa chain]: Mitochondrion matrix . Produced by the proteolytic processing of the 74-kDa cytosolic precursor. .
Tissue Expression	Isoform 1 and isoform 3 are detected in brain cortex. Isoform 3 is highly expressed in astrocytoma, ganglioglioma and ependymoma. Isoform 1 is highly expressed in brain and kidney, but not detected in liver. Isoform 3 is highly expressed in heart and pancreas, detected at lower levels in placenta, lung, pancreas and kidney, but is not detected in liver. Isoform 2 is expressed in cardiac and skeletal muscle.
Signaling_pathway	Organismal Systems >> Nervous system >> Glutamatergic synapse;Human Diseases >> Cancer: overview >> MicroRNAs in cancer;Human Diseases >> Cancer: overview >> Central carbon metabolism in cancer
Research Areas	>>Arginine biosynthesis; / >>Alanine, aspartate and glutamate metabolism; / >>D-Amino acid metabolism; / >>Metabolic pathways; / >>Glutamatergic synapse; / >>GABAergic synapse; / >>Proximal tubule bicarbonate reclamation; / >>MicroRNAs in cancer; / >>Central carbon metabolism in cancer
Function	Catalytic activity:L-glutamine + H(2)O = L-glutamate + NH(3).,Function:Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine.,similarity:Belongs to the glutaminase family.,similarity:Contains 1 ANK repeat.,tissue specificity:KGA is expressed predominantly in brain and kidney but not in liver, GAC is expressed principally in cardiac muscle and pancreas but not in liver or brain, and GAM is expressed solely in cardiac and skeletal muscle.,
RRID