Name: GluR-1 Polyclonal Antibody
SKU: WP12489
Price: 268 USD
Availability: InStock

Product details

Background:

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

Specifications

Target	GluR-1
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, ELISA
MW(Calculated)
MW(Observed)	95kD
Host Species	Rabbit
Isotype	IgG
Conjugate/Modification	Unmodified
Modification
Recommended Dilution Ratio	WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:40000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#YS0004)
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration	1 mg/ml
Clonality	Polyclonal
Clone Number
Immunogen	The antiserum was produced against synthesized peptide derived from human GluR1. AA range:816-865
Sequence
Specificity	Glutamate Receptor 1 Polyclonal Antibody detects endogenous levels of Glutamate Receptor 1 protein.
Gene Name	GRIA1
Protein Name	Glutamate receptor 1
Other Name	GRIA1; / GLUH1; / GLUR1; / Glutamate receptor 1; / GluR-1; / AMPA-selective glutamate receptor 1; / GluR-A; / GluR-K1; / Glutamate receptor ionotropic; / AMPA 1; / GluA1
Species	Human
Gene ID-1	2890
Uniprot	P42261,
Species.1	Mouse
Gene ID-2	14799
Uniprot.1	P23818,
Species.2	Rat
Gene ID-3	50592
Uniprot.2	P19490
Organism-4
Gene ID-4
SwissProt-4
Background	Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
Cellular Localization	Cell membrane; Multi-pass membrane protein . Endoplasmic reticulum membrane; Multi-pass membrane protein . Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein . Cell junction, synapse, postsynaptic density membrane; Multi-pass membrane protein . Cell projection, dendrite . Cell projection, dendritic spine . Early endosome membrane; Multi-pass membrane protein . Recycling endosome membrane; Multi-pass membrane protein . Cell junction, synapse, presynapse . Cell junction, synapse . Interaction with CACNG2, CNIH2 and CNIH3 promotes cell surface expression. Colocalizes with PDLIM4 in early endosomes. Displays a somatodendritic localization and is excluded from axons in neurons (By similarity). Localized to cone photoreceptor pedicles (By similarity). .
Tissue Expression	Widely expressed in brain.
Signaling_pathway	Organismal Systems >> Nervous system >> Glutamatergic synapse;Organismal Systems >> Nervous system >> Dopaminergic synapse;Human Diseases >> Neurodegenerative disease >> Amyotrophic lateral sclerosis;Human Diseases >> Neurodegenerative disease >> Huntington disease;Human Diseases >> Neurodegenerative disease >> Spinocerebellar ataxia;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases;Environmental Information Processing >> Signal transduction >> cAMP signaling pathway
Research Areas	>>cAMP signaling pathway; / >>Neuroactive ligand-receptor interaction; / >>Circadian entrainment; / >>Long-term potentiation; / >>Retrograde endocannabinoid signaling; / >>Glutamatergic synapse; / >>Dopaminergic synapse; / >>Long-term depression; / >>Amyotrophic lateral sclerosis; / >>Huntington disease; / >>Spinocerebellar ataxia; / >>Pathways of neurodegeneration - multiple diseases; / >>Amphetamine addiction; / >>Nicotine addiction
Function	Function:Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.,miscellaneous:The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds AMPA (quisqualate) > glutamate > kainate.,PTM:Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.,similarity:Belongs to the glutamate-gated ion channel (TC 1.A.10) family.,subcellular location:Interaction with CACNG2 promotes cell surface expression.,subunit:Homotetramer or heterotetramer of pore-forming glutamate receptor subunits. Tetramers may be formed by the dimerization of dimers. Interacts with DLG1 via its C-terminus. Interacts with CACNG2, HIP1 and RASGRF2.,tissue specificity:Widely expressed in brain.,
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GluR-1 Polyclonal Antibody

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GluR-1 Polyclonal Antibody

Product details

Specifications

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