Name: GluR4 Polyclonal Antibody
SKU: WP12491
Price: 268 USD
Availability: InStock

Product details

Background:

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes composed of multiple subunits, arranged to form ligand-gated ion channels. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. The subunit encoded by this gene belongs to a family of AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate)-sensitive glutamate receptors, and is subject to RNA editing (AGA->GGA; R->G). Alternative splicing of this gene results in transcript variants encoding different isoforms, which may vary in their signal transduction properties. Some haplotypes of this gene show a positive association with schizophrenia. [provided by RefSeq, Jul 2008],

Specifications

Size	50μL/100μL
Target	GluR4
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, ELISA
MW(Calculated)
MW(Observed)	100kD
Host Species	Rabbit
Isotype	IgG
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration	1 mg/ml
Clonality	Polyclonal
Clone Number
Immunogen	The antiserum was produced against synthesized peptide derived from human GluR4. AA range:828-877
Specificity	GluR4 Polyclonal Antibody detects endogenous levels of GluR4 protein.
Gene Name	GRIA4
Protein Name	Glutamate receptor 4
Other Name	GRIA4; / GLUR4; / Glutamate receptor 4; / GluR-4; / GluR4; / AMPA-selective glutamate receptor 4; / GluR-D; / Glutamate receptor ionotropic; / AMPA 4; / GluA4
Species	Human
Gene ID	2893
Uniprot	P48058,
Species.1	Mouse
Gene ID.1	14802
Uniprot.1	Q9Z2W8,
Species.2	Rat
Gene ID.2	29629
Uniprot.2	P19493
Cellular Localization	Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell projection, dendrite. Interaction with CNIH2, CNIH3 and PRKCG promotes cell surface expression. .
Tissue Expression	Brain,Donated clones,PCR rescued clones,
Signaling_pathway	Organismal Systems >> Nervous system >> Glutamatergic synapse;Organismal Systems >> Nervous system >> Dopaminergic synapse;Human Diseases >> Neurodegenerative disease >> Huntington disease;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases;Environmental Information Processing >> Signal transduction >> cAMP signaling pathway
Research Areas	>>cAMP signaling pathway; / >>Neuroactive ligand-receptor interaction; / >>Circadian entrainment; / >>Retrograde endocannabinoid signaling; / >>Glutamatergic synapse; / >>Dopaminergic synapse; / >>Huntington disease; / >>Pathways of neurodegeneration - multiple diseases; / >>Amphetamine addiction; / >>Nicotine addiction
Function	Function:Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.,miscellaneous:The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds AMPA (quisqualate) > glutamate > kainate.,PTM:Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-611 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-837 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.,similarity:Belongs to the glutamate-gated ion channel (TC 1.A.10) family.,subunit:Homotetramer or heterotetramer of pore-forming glutamate receptor subunits. Tetramers may be formed by the dimerization of dimers. Interacts with EPB41L1 via its C-terminus.,