Glut4 Polyclonal Antibody
Ucallm_WP15537-50
Price range: $268.00 through $328.00
Product details
Background:
This gene is a member of the solute carrier family 2 (facilitated glucose transporter) family and encodes a protein that functions as an insulin-regulated facilitative glucose transporter. In the absence of insulin, this integral membrane protein is sequestered within the cells of muscle and adipose tissue. Within minutes of insulin stimulation, the protein moves to the cell surface and begins to transport glucose across the cell membrane. Mutations in this gene have been associated with noninsulin-dependent diabetes mellitus (NIDDM). [provided by RefSeq, Jul 2008],
Specifications
| Size | 50μL/100μL |
| Target | Glut4 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 56kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC: 1:100-300; ELISA 1:20000; IF 1:100-300; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from the N-terminal region of human SLC2A4. AA range:21-70 |
| Specificity | Glut4 Polyclonal Antibody detects endogenous levels of Glut4 protein. |
| Gene Name | SLC2A4 |
| Protein Name | Solute carrier family 2 facilitated glucose transporter member 4 |
| Other Name | SLC2A4; / GLUT4; / Solute carrier family 2, facilitated glucose transporter member 4; / Glucose transporter type 4, insulin-responsive; / GLUT-4 |
| Species | Human |
| Gene ID | 6517 |
| Uniprot | P14672, |
| Species.1 | Mouse |
| Gene ID.1 | 20528 |
| Uniprot.1 | P14142, |
| Species.2 | Rat |
| Gene ID.2 | 25139 |
| Uniprot.2 | P19357 |
| Cellular Localization | Cell membrane; Multi-pass membrane protein . Endomembrane system; Multi-pass membrane protein . Cytoplasm, perinuclear region . Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized (PubMed:8300557). The dileucine internalization motif is critical for intracellular sequestration (PubMed:8300557). Insulin stimulation induces translocation to the cell membrane (By similarity). . |
| Tissue Expression | Skeletal and cardiac muscles; brown and white fat. |
| Signaling_pathway | Organismal Systems >> Endocrine system >> Insulin signaling pathway;Organismal Systems >> Endocrine system >> Adipocytokine signaling pathway;Environmental Information Processing >> Signal transduction >> FoxO signaling pathway;Environmental Information Processing >> Signal transduction >> AMPK signaling pathway |
| Research Areas | >>FoxO signaling pathway; / >>AMPK signaling pathway; / >>Insulin signaling pathway; / >>Adipocytokine signaling pathway; / >>Type II diabetes mellitus; / >>Insulin resistance; / >>Diabetic cardiomyopathy |
| Function | Disease:Defects in SLC2A4 may be a cause of noninsulin-dependent diabetes mellitus (NIDDM) [MIM:125853]. Defects in SLC2A4 may be a cause of certain post-receptor defects in NIDDM. The variant in position Ile-383 is found in a small number of NIDDM patients, but seems not to be found in nondiabetic subjects.,Function:Insulin-regulated facilitative glucose transporter.,miscellaneous:Insulin-stimulated phosphorylation of TBC1D4 is required for GLUT4 translocation.,online information:GLUT4 entry,PTM:Sumoylated.,similarity:Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.,subcellular location:Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized. The dileucine internalization motif is critical for intracellular sequestration.,subunit:Binds to DAXX. Interacts via its N-terminus with SRFBP1.,tissue specificity:Skeletal and cardiac muscles; brown and white fat., |
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