U-Blot® Glutamate Receptor 1 Rabbit mAb
WR4579 Mouse brain whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with primary antibody(1:1000). The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.
Price range: $268.00 through $328.00
Product details
Background:Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
Specifications
| Size | 50μL/100μL |
| Target | GluR-1 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 101kD |
| MW(Observed) | 101kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:1000-1:5000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0846R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | GRIA1 |
| Protein Name | Glutamate receptor 1 |
| Other Name | GRIA1; / GLUH1; / GLUR1; / Glutamate receptor 1; / GluR-1; / AMPA-selective glutamate receptor 1; / GluR-A; / GluR-K1; / Glutamate receptor ionotropic; / AMPA 1; / GluA1 |
| Species | Human |
| Gene ID | 2890 |
| Uniprot | P42261, |
| Species.1 | Mouse |
| Gene ID.1 | 14799 |
| Uniprot.1 | P23818, |
| Species.2 | Rat |
| Gene ID.2 | 50592 |
| Uniprot.2 | P19490 |
| Cellular Localization | Cell membrane; Multi-pass membrane protein . Endoplasmic reticulum membrane; Multi-pass membrane protein . Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein . Cell junction, synapse, postsynaptic density membrane; Multi-pass membrane protein . Cell projection, dendrite . Cell projection, dendritic spine . Early endosome membrane; Multi-pass membrane protein . Recycling endosome membrane; Multi-pass membrane protein . Cell junction, synapse, presynapse . Cell junction, synapse . Interaction with CACNG2, CNIH2 and CNIH3 promotes cell surface expression. Colocalizes with PDLIM4 in early endosomes. Displays a somatodendritic localization and is excluded from axons in neurons (By similarity). Localized to cone photoreceptor pedicles (By similarity). . |
| Tissue Expression | Widely expressed in brain. |
| Signaling_pathway | Organismal Systems >> Nervous system >> Glutamatergic synapse;Organismal Systems >> Nervous system >> Dopaminergic synapse;Human Diseases >> Neurodegenerative disease >> Amyotrophic lateral sclerosis;Human Diseases >> Neurodegenerative disease >> Huntington disease;Human Diseases >> Neurodegenerative disease >> Spinocerebellar ataxia;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases;Environmental Information Processing >> Signal transduction >> cAMP signaling pathway |
| Research Areas | >>cAMP signaling pathway; / >>Neuroactive ligand-receptor interaction; / >>Circadian entrainment; / >>Long-term potentiation; / >>Retrograde endocannabinoid signaling; / >>Glutamatergic synapse; / >>Dopaminergic synapse; / >>Long-term depression; / >>Amyotrophic lateral sclerosis; / >>Huntington disease; / >>Spinocerebellar ataxia; / >>Pathways of neurodegeneration - multiple diseases; / >>Amphetamine addiction; / >>Nicotine addiction |
| Function | Function:Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.,miscellaneous:The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds AMPA (quisqualate) > glutamate > kainate.,PTM:Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.,similarity:Belongs to the glutamate-gated ion channel (TC 1.A.10) family.,subcellular location:Interaction with CACNG2 promotes cell surface expression.,subunit:Homotetramer or heterotetramer of pore-forming glutamate receptor subunits. Tetramers may be formed by the dimerization of dimers. Interacts with DLG1 via its C-terminus. Interacts with CACNG2, HIP1 and RASGRF2.,tissue specificity:Widely expressed in brain., |
Please login to post questions
Related products
