Name: U-Blot® Glutaminase C Rabbit mAb
SKU: WR4082
Price: 268 USD
Availability: InStock

U-Blot® Glutaminase C Rabbit mAb

WR4082

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).

WR4082 Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).

Western blot analysis of lysates from Hela cells, using Glutaminase C Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.

WR4082 Western blot analysis of lysates from Hela cells, using Glutaminase C Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.

Price range: $268.00 through $328.00

Size

100μl

50μl

Clear

SKU: WR4082-50

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Product details

Background:This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012],

Specifications

Size	50μL/100μL
Target	GLSK
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, IP, ELISA
MW(Calculated)	68kD
MW(Observed)	55-65kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:200-1000; WB 1:1000-5000; IF 1:200-1000; ELISA 1:5000-20000; IP 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM0168R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	GLS GLS1 KIAA0838
Protein Name	GLSK
Other Name
Species	Human
Gene ID	2744
Uniprot	O94925,
Species.1	Mouse
Gene ID.1	14660
Uniprot.1	D3Z7P3,
Species.2	Rat
Gene ID.2	24398
Uniprot.2	P13264
Cellular Localization	Cytoplasm
Tissue Expression	Isoform 1 and isoform 3 are detected in brain cortex. Isoform 3 is highly expressed in astrocytoma, ganglioglioma and ependymoma. Isoform 1 is highly expressed in brain and kidney, but not detected in liver. Isoform 3 is highly expressed in heart and pancreas, detected at lower levels in placenta, lung, pancreas and kidney, but is not detected in liver. Isoform 2 is expressed in cardiac and skeletal muscle.
Signaling_pathway	Organismal Systems >> Nervous system >> Glutamatergic synapse;Human Diseases >> Cancer: overview >> MicroRNAs in cancer;Human Diseases >> Cancer: overview >> Central carbon metabolism in cancer
Research Areas	>>Arginine biosynthesis; / >>Alanine, aspartate and glutamate metabolism; / >>D-Amino acid metabolism; / >>Metabolic pathways; / >>Glutamatergic synapse; / >>GABAergic synapse; / >>Proximal tubule bicarbonate reclamation; / >>MicroRNAs in cancer; / >>Central carbon metabolism in cancer
Function	Catalytic activity:L-glutamine + H(2)O = L-glutamate + NH(3).,Function:Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine.,similarity:Belongs to the glutaminase family.,similarity:Contains 1 ANK repeat.,tissue specificity:KGA is expressed predominantly in brain and kidney but not in liver, GAC is expressed principally in cardiac muscle and pancreas but not in liver or brain, and GAM is expressed solely in cardiac and skeletal muscle.,