Price range: $268.00 through $328.00
Background:
Glypican 3 is a carcinoembryonic protein and belongs to the heparan sulfate polysaccharide family. It is mainly expressed in fetal liver, kidney and trophoblast, but not in other normal tissues. Glypican 3 is a sensitive and specific marker of hepatocellular carcinoma. Its expression is related to differentiation. The lower the degree of differentiation, the higher the expression. It is highly expressed in ovarian yolk sac tumor, choriocarcinoma and melanoma.
| Size | 50μL/100μL |
| Target | Glypican-3 |
| Reactivity | Human |
| Applications | IHC, WB, IF, ELISA |
| MW(Calculated) | 66kD |
| MW(Observed) | 66kD,58kD,22kD |
| Host Species | Mouse |
| Isotype | IgG1,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1000; WB 1:500-2000; IF 1:100-500; ELISA 1:1000-5000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein G affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RMM068 |
| Immunogen | Synthesized peptide derived from human Glypican-3(GPC3) AA range: 400-500 |
| Specificity | The antibody can specifically recognize human Glypican-3 protein. |
| Gene Name | GPC3 OCI5 |
| Protein Name | Glypican-3(GPC3) |
| Other Name | DGSX; / Glypican proteoglycan 3; / Glypican-3 [Precursor]; / Gpc3; / GPC3_HUMAN; / GTR2 2; / GTR2-2; / Heparan sulphate proteoglycan; / Intestinal protein OCI 5; / Intestinal protein OCI-5; / MXR7; / OCI 5; / OCI-5; / OCI5; / SDYS; / Secreted glypican-3; / SGB; / SGBS; / SGBS1 |
| Species | Human |
| Gene ID | 2719 |
| Uniprot | P51654 |
| Species.1 | |
| Gene ID.1 | |
| Uniprot.1 | |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasmic |
| Tissue Expression | Highly expressed in lung, liver and kidney. |
| Signaling_pathway | |
| Research Areas | >>Proteoglycans in cancer |
| Function | Disease:Defects in GPC3 are the cause of Simpson-Golabi-Behmel syndrome (SGBS) [MIM:312870]; also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies.,Function:Cell surface proteoglycan that bears heparan sulfate.,Function:Cell surface proteoglycan that bears heparan sulfate. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition.,similarity:Belongs to the glypican family.,tissue specificity:Highly expressed in lung, liver and kidney., |
