U-Blot® Histone H2B (Acetyl Lys126) Rabbit pAb

Price range: $288.00 through $368.00

SKU: WP0274-100
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Product details

Background:Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a testis/sperm-specific member of the histone H2B family. Transcripts from this gene contain a palindromic termination element. [provided by RefSeq, Aug 2015],

Specifications

TargetHistone H2B
ReactivityHuman,Mouse,Rat
ApplicationWB,ELISA
MW(Calculated)
MW(Observed)15kD
Host SpeciesRabbit
IsotypeIgG
ConjugateUnconjugated
ModificationAcetyl
Modification siteLys126
Recommended dilution ratioWB 1:500-1:2000;ELISA 1:20000;Not yet tested in other applications.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenThe antiserum was produced against synthesized Acetyl-peptide derived from human H2B around the Acetylation site of Lys126. AA range:78-127
Sequence
SpecificityThis antibody detects histone H2A.Z only when it is L-lactylated at Lys11. detects endogenous levels of Histone H2B protein only when acetylated at K126.
Gene nameHIST1H2BC
Protein nameHistone H2B type 1-A/Histone H2B type 1-B/Histone H2B type 1-C/E/F/G/I
Alternative nameHIST1H2BA;TSH2B;Histone H2B type 1-A;Histone H2B, testis;Testis-specific histone H2B;HIST1H2BB;H2BFF;Histone H2B type 1-B;Histone H2B.1;Histone H2B.f;H2B/f;HIST1H2BC;H2BFL;HIST1H2BE;H2BFH;HIST1H2BF;H2BFG;HIST1H2BG;H2BFA;HIST1H2BI;H2BFK;Histone H2B type 1-C/E/F/G/I;Histone H2B.1 A;Histone H2B.a;H2B/a;Histone H2B.g;H2B/g;Histone H2B.h;H2B/h;Histone H2B.k;H2B/k;Histone H2B.l;H2B/l;H2BK126AC
Organism-1Human
Gene ID‑1255626
SwissProt-1Q96A08
Organism-2Mouse
Gene ID‑2319177
SwissProt-2P70696
Organism-3Rat
Gene ID‑324829
SwissProt-3Q00729
Organism-4
Gene ID‑4
SwissProt-4
Cellular LocalizationNucleus . Chromosome .
Unnamed: 38
Unnamed: 39
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