Name: U-Blot® Histone H2B (Tri Methyl Lys43) Rabbit pAb
SKU: WP0243
Price: 288 USD
Availability: InStock

Product details

Background:

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a testis/sperm-specific member of the histone H2B family. Transcripts from this gene contain a palindromic termination element. [provided by RefSeq, Aug 2015],

Specifications

Target	Histone H2B Tri Methyl Lys43
Reactivity	Human, Mouse, Rat
Applications	WB
MW(Calculated)
MW(Observed)	14kD
Host Species	Rabbit
Isotype	IgG
Conjugate/Modification	trimethylate
Modification
Recommended Dilution Ratio	WB 1:500-1000
Formulation	PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Source
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.
Purity
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Polyclonal
Clone Number
Immunogen	Synthetic Peptide of Histone H2B (Tri Methyl Lys43)
Sequence
Specificity	The antibody detects endogenous Histone H2B (Tri Methyl Lys43) protein.
Gene Name	HIST1H2BC
Protein Name	Histone H2B type 1-A/Histone H2B type 1-B/Histone H2B type 1-C/E/F/G/I
Other Name	H2BK43ME3; / HIST1H2BA; / TSH2B; / Histone H2B type 1-A; / Histone H2B, testis; / Testis-specific histone H2B; / HIST1H2BB; / H2BFF; / Histone H2B type 1-B; / Histone H2B.1; / Histone H2B.f; / H2B/f; / HIST1H2BC; / H2BFL; / HIST1H2BE; / H2BFH; / HIST1H2BF; / H2BFG; / HIST1H2BG; / H2BFA; / HIST1H2BI; / H2BFK; / Histone H2B type 1-C/E/F/G/I; / Histone H2B.1 A; / Histone H2B.a; / H2B/a; / Histone H2B.g; / H2B/g; / Histone H2B.h; / H2B/h; / Histone H2B.k; / H2B/k; / Histone H2B.l; / H2B/l
Species	Human
Gene ID-1	255626;3018;3017;8339;8343;8344;8346;8347
Uniprot	Q96A08;P33778;P62807,
Species.1	Mouse
Gene ID-2	319177;319178;319179,
Uniprot.1
Species.2	Rat
Gene ID-3	24829
Uniprot.2	Q00729
Organism-4
Gene ID-4
SwissProt-4
Background	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a testis/sperm-specific member of the histone H2B family. Transcripts from this gene contain a palindromic termination element. [provided by RefSeq, Aug 2015],
Cellular Localization	Nucleus . Chromosome .
Tissue Expression	Mainly expressed in testis, and the corresponding protein is also present in mature sperm (at protein level). Also found in some fat cells.
Signaling_pathway	Organismal Systems >> Immune system >> Neutrophil extracellular trap formation;Human Diseases >> Immune disease >> Systemic lupus erythematosus
Research Areas	>>Neutrophil extracellular trap formation; / >>Alcoholism; / >>Viral carcinogenesis; / >>Systemic lupus erythematosus
Function	Function:Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.,PTM:Monoubiquitination of Lys-122 by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II.,similarity:Belongs to the histone H2B family.,subunit:The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.,tissue specificity:Transcribed exclusively in testis, and the corresponding protein is also present in mature sperm.,
RRID

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U-Blot® Histone H2B (Tri Methyl Lys43) Rabbit pAb

Product details

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U-Blot® Histone H2B (Tri Methyl Lys43) Rabbit pAb

Product details

Specifications

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