Name: U-Blot® Histone H3 (Di Methyl Lys4) Rabbit mAb
SKU: WR4514
Price: 288 USD
Availability: InStock

U-Blot® Histone H3 (Di Methyl Lys4) Rabbit mAb

Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4°C, over night with a 1:5000 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody(Cat:WS0446) was used to detect the antibody.

WR4514 Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4°C, over night with a 1:5000 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody(Cat:WS0446) was used to detect the antibody.

NIH-3T3 cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with primary antibody 1:1000. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.

WR4514 NIH-3T3 cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with primary antibody 1:1000. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.

Price range: $288.00 through $368.00

Size

100μl

50μl

Clear

SKU: WR4514-50

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Product details

Background:Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015],

Specifications

Target	Histone H3
Reactivity	Human,Mouse,Rat
Applications	WB,IHC,IF,IP,ELISA,CHIP,Cut&Tag
MW(Calculated)	15kD
MW(Observed)	17kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Di Methyl
Modification Site	Lys4
Recommended Dilution Ratio	IHC 1:1000-1:5000;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200;CHIP 1:50-1:100;Cut&Tag 1:50-1:100;
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Source
Purification	Protein A
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Clonality	Monoclonal
Clone Number	PT0736R
Specificity	Di-Methyl-Histone H3 (K4) Antibody detects endogenous levels of Histone H3 protein only when di-methylated at K4.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):RTkQT
Gene Name	HIST1H3A/HIST1H3/HIST1H3C/HIST1H3D/HIST1H3E/HIST1H3F/HIST1H3G/HIST1H3H/HIST1H3I/HIST1H3J/HIST2H3A/HIST2H3C/HIST2H3D/H3F3A/H3F3B/H3F3C
Protein Name	Histone H3.1/Histone H3.2/Histone H3.3/Histone H3.3C
Other Name	H3K4ME2;HIST1H3A;H3FA;HIST1H3B;H3FL;HIST1H3C;H3FC;HIST1H3D;H3FB;HIST1H3E;H3FD;HIST1H3F;H3FI;HIST1H3G;H3FH;HIST1H3H;H3FK;HIST1H3I;H3FF;HIST1H3J;H3FJ;Histone H3.1;Histone H3.1;Histone H3.2;Histone H3/m;Histone H3/o;H3F3A;H3.3A;H3F3;PP781;H3F3B;H3.3B;Histone H3.3;H3F3C;Histone H3.3C;Histone H3.5
Organism-1	Human
Gene ID-1	8350;8351;8352;8353;8354;8355;8356;8357;8358;8968;126961;333932;653604;3020;3021;440093
SwissProt-1	P68431;Q71DI3;P84243;Q6NXT2
Organism-2	Mouse
Gene ID-2	319152;15077;15078;625328
SwissProt-2
Organism-3	Rat
Gene ID-3	291159;100361558
SwissProt-3	Q6LED0;P84245
Organism-4
GeneID-4
SwissProt-4
Cellular Localization	Nucleus. Chromosome.
Unnamed: 33