Product details
The WB blocking solution is designed to block non-specific binding of antibodies to WB blot membranes. This proprietary formulated blocker is non-casein and non-milk based solution that effectively blocks non-specific binding rapidly and effectively resulting in clear background, increased contrast and enhanced signal intensity. This solution can also be used in ELISA, IHC and other detection system where blocking of non-specific antibody binding is needed. This blocking solution does not contain detergent and Tris.
Use and Procedures
Add the blocking solution to blotting membrane (nitrocellulose, PVDF or other), ELISA well, and sample spot on IHC slide. Incubate 30 min to 2h at room temperature or overnight at 4℃. After blocking, rinse the blotting membrane once with ddH2O and continue with the other steps of your experiment. This solution can also be used as a diluent for primary antibody but it is not recommended for dilution of peroxidase- conjugated secondary antibodies.
