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Home/Reagents/Antibody/Unmodified Histone Antibodies/Unmodified Histone Antibodies/U-Blot® Histone H4 Rabbit mAb
SKU: WR4068 Categories: , ,

U-Blot® Histone H4 Rabbit mAb

Ucallm_WR4068-50
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
WR4068 Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
Western blot analysis of lysates from Hela cells, using Histone H4 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.
WR4068 Western blot analysis of lysates from Hela cells, using Histone H4 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.

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SKU: WR4068-50
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Product details

Background:Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the histone microcluster on chromosome 6p21.33. [provided by RefSeq, Aug 2015],

Specifications

TargetHistone H4
ReactivityHuman, Mouse, Rat
ApplicationsWB, IHC, IF, IP, ELISA, CHIP, Cut&Tag
MW(Calculated)11kD
MW(Observed)11kD
Host SpeciesRabbit
IsotypeIgG,Kappa
Conjugate/ModificationUnmodified
Modification
Recommended Dilution RatioIHC 1:200-1000; WB 1:1000-5000; IF 1:200-1000; ELISA 1:5000-20000; IP 1:50-200; CHIP 1:50-1:100; Cut&Tag 1:50-1:100; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#YS0004)
FormulationPBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Source
PurificationRecombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Purity
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
ClonalityMonoclonal
Clone NumberPT0151R
ImmunogenThe specific immunogen used to produce this antibody is proprietary information.
Sequence
SpecificityEndogenous
Gene NameHIST1H4A/HIST1H4B/HIST1H4C/HIST1H4D/HIST1H4E/HIST1H4F/HIST1H4H/HIST1H4I/HIST1H4J/HIST1H4K/HIST1H4L/HIST2H4A/HIST2H4B/HIST4H4
Protein NameHistone H4
Other NameHIST1H4A; / H4/A; / H4FA; / HIST1H4B; / H4/I; / H4FI; / HIST1H4C; / H4/G; / H4FG; / HIST1H4D; / H4/B; / H4FB; / HIST1H4E; / H4/J; / H4FJ; / HIST1H4F; / H4/C; / H4FC; / HIST1H4H; / H4/H; / H4FH; / HIST1H4I; / H4/M; / H4FM; / HIST1H4J; / H4/E; / H4FE; / HIST1H4K; / H4/D; / H4FD; / HIST1H4L; / H4/K; / H4FK
SpeciesHuman
Gene ID-1121504;554313;8294;8359;8360;8361;8362;8363;8364;8365;8366;8367;8368;8370
UniprotP62805,
Species.1Mouse
Gene ID-2100041230
Uniprot.1P62806,
Species.2Rat
Gene ID-3100360950
Uniprot.2P62804
Organism-4
Gene ID-4
SwissProt-4
BackgroundHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the histone microcluster on chromosome 6p21.33. [provided by RefSeq, Aug 2015],
Cellular LocalizationNucleus. Chromosome.
Tissue ExpressionB-cell lymphoma,Bone marrow,Brain,Clones donated by HIP,Corpus call
Signaling_pathwayOrganismal Systems >> Immune system >> Neutrophil extracellular trap formation;Human Diseases >> Immune disease >> Systemic lupus erythematosus
Research Areas>>Neutrophil extracellular trap formation; / >>Alcoholism; / >>Viral carcinogenesis; / >>Systemic lupus erythematosus
FunctionFunction:Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.,PTM:Acetylation at Lys-6, Lys-9, Lys-13 and Lys-17 occurs in coding regions of the genome but not in heterochromatin.,PTM:Citrullination at Arg-4 by PADI4 impairs methylation.,PTM:Monomethylated, dimethylated or trimethylated at Lys-21. Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.,PTM:Monomethylation at Arg-4 by PRMT1 favors acetylation at Lys-9 and Lys-13. Demethylation is performed by JMJD6.,PTM:Sumoylated, which is associated with transcriptional repression.,PTM:Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.,similarity:Belongs to the histone H4 family.,subunit:The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.,
RRID
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