Name: HXK I Monoclonal Antibody
SKU: WM0246
Price: 268 USD
Availability: InStock

Product details

Background:
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific. [provided by RefSeq, Apr 2016],

Specifications

Size	50μL/100μL
Target	HXK I
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, FC, ELISA
MW(Calculated)	102kD
MW(Observed)
Host Species	Mouse
Isotype
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	WB 1:500-1:2000; IHC 1:200-1:1000; IF 1:200-1:1000; Flow Cyt 1:200-1:400; ELISA 1:10000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification	Affinity purification
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	14H1
Immunogen	Purified recombinant fragment of human HXK I expressed in E. Coli.
Specificity	HXK I Monoclonal Antibody detects endogenous levels of HXK I protein.
Gene Name	HK1
Protein Name	Hexokinase-1
Other Name	HK1; / Hexokinase-1; / Brain form hexokinase; / Hexokinase type I; / HK I
Species	Human
Gene ID	3098
Uniprot	P19367,
Species.1	Mouse
Gene ID.1
Uniprot.1	P17710,
Species.2	Rat
Gene ID.2	25058
Uniprot.2	P05708
Cellular Localization	Mitochondrion outer membrane; Peripheral membrane protein . Cytoplasm, cytosol . The mitochondrial-binding peptide (MBP) region promotes association with the mitochondrial outer membrane (Probable). Dissociates from the mitochondrial outer membrane following inhibition by N-acetyl-D-glucosamine, leading to relocation to the cytosol (PubMed:27374331). .
Tissue Expression	Isoform 2: Erythrocyte specific (Ref.6). Isoform 3: Testis-specific (PubMed:10978502). Isoform 4: Testis-specific (PubMed:10978502).
Signaling_pathway	Organismal Systems >> Endocrine system >> Insulin signaling pathway;Human Diseases >> Cancer: overview >> Central carbon metabolism in cancer;Environmental Information Processing >> Signal transduction >> HIF-1 signaling pathway
Research Areas	>>Glycolysis / Gluconeogenesis; / >>Fructose and mannose metabolism; / >>Galactose metabolism; / >>Starch and sucrose metabolism; / >>Amino sugar and nucleotide sugar metabolism; / >>Neomycin, kanamycin and gentamicin biosynthesis; / >>Metabolic pathways; / >>Carbon metabolism; / >>Biosynthesis of nucleotide sugars; / >>HIF-1 signaling pathway; / >>Insulin signaling pathway; / >>Type II diabetes mellitus; / >>Carbohydrate digestion and absorption; / >>Shigellosis; / >>Central carbon metabolism in cancer
Function	Catalytic activity:ATP + D-hexose = ADP + D-hexose 6-phosphate.,Disease:Defects in HK1 are the cause of hexokinase deficiency [MIM:235700]. Hexokinase deficiency is a rare autosomal recessive disease with nonspherocytic hemolytic anemia as the predominant clinical feature.,Domain:The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus.,enzyme regulation:Hexokinase is an allosteric enzyme inhibited by its product Glc-6-P.,miscellaneous:In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase).,online information:Hexokinase entry,pathway:Carbohydrate metabolism; hexose metabolism.,similarity:Belongs to the hexokinase family.,subcellular location:Its hydrophobic N-terminal sequence may be involved in membrane binding.,subunit:Monomer.,tissue specificity:Isoform 2 is erythrocyte specific; isoform 3 and isoform 4 are testis-specific.,

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HXK I Monoclonal Antibody

Product details

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HXK I Monoclonal Antibody

Product details

Specifications

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