IκB-α Monoclonal Antibody
Ucallm_WM0504-50
Price range: $268.00 through $328.00
Product details
Background:
This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011],
Specifications
| Size | 50μL/100μL |
| Target | IκB-α |
| Reactivity | Human, Mouse, Rat, Pig, sheep |
| Applications | WB |
| MW(Calculated) | 36kD |
| MW(Observed) | |
| Host Species | Mouse |
| Isotype | |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:1000-1:2000; Not yet tested in other applications. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | Affinity purification |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | 20G7 |
| Immunogen | Purified recombinant human IκB-α (N-terminus) protein fragments expressed in E.coli. |
| Specificity | IκB-α Monoclonal Antibody detects endogenous levels of IκB-α protein. |
| Gene Name | NFKBIA IKBA MAD3 NFKBI |
| Protein Name | NF-kappa-B inhibitor alpha |
| Other Name | NFKBIA; / IKBA; / MAD3; / NFKBI; / NF-kappa-B inhibitor alpha; / I-kappa-B-alpha; / IkB-alpha; / IkappaBalpha; / Major histocompatibility complex enhancer-binding protein MAD3 |
| Species | Human |
| Gene ID | 4792 |
| Uniprot | P25963, |
| Species.1 | Mouse |
| Gene ID.1 | 18035 |
| Uniprot.1 | Q9Z1E3, |
| Species.2 | Rat |
| Gene ID.2 | 25493 |
| Uniprot.2 | Q63746 |
| Cellular Localization | Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export. . |
| Tissue Expression | Brain,Kidney,Lymph node,Monocyte, |
| Signaling_pathway | Cellular Processes >> Cell growth and death >> Apoptosis;Organismal Systems >> Immune system >> Toll-like receptor signaling pathway;Organismal Systems >> Immune system >> NOD-like receptor signaling pathway;Organismal Systems >> Immune system >> RIG-I-like receptor signaling pathway;Organismal Systems >> Immune system >> Cytosolic DNA-sensing pathway;Organismal Systems >> Immune system >> T cell receptor signaling pathway;Organismal Systems >> Immune system >> Th1 and Th2 cell differentiation;Organismal Systems >> Immune system >> Th17 cell differentiation;Organismal Systems >> Immune system >> IL-17 signaling pathway;Organismal Systems >> Immune system >> B cell receptor signaling pathway;Organismal Systems >> Immune system >> Chemokine signaling pathway;Organismal Systems >> Endocrine system >> Adipocytokine signaling pathway;Organismal Systems >> Endocrine system >> Relaxin signaling pathway;Organismal Systems >> Nervous system >> Neurotrophin signaling pathway;Organismal Systems >> Development and regeneration >> Osteoclast differentiation;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: overview >> PD-L1 expression and PD-1 checkpoint pathway in cancer;Human Diseases >> Cancer: specific types >> Chronic myeloid leukemia;Human Diseases >> Cancer: specific types >> Prostate cancer;Human Diseases >> Cancer: specific types >> Small cell lung cancer;Environmental Information Processing >> Signal transduction >> NF-kappa B signaling pathway;Environmental Information Processing >> Signal transduction >> TNF signaling pathway;Environmental Information Processing >> Signal transduction >> cAMP signaling pathway |
| Research Areas | >>cAMP signaling pathway; / >>Chemokine signaling pathway; / >>NF-kappa B signaling pathway; / >>Apoptosis; / >>Osteoclast differentiation; / >>Toll-like receptor signaling pathway; / >>NOD-like receptor signaling pathway; / >>RIG-I-like receptor signaling pathway; / >>Cytosolic DNA-sensing pathway; / >>C-type lectin receptor signaling pathway; / >>IL-17 signaling pathway; / >>Th1 and Th2 cell differentiation; / >>Th17 cell differentiation; / >>T cell receptor signaling pathway; / >>B cell receptor signaling pathway; / >>TNF signaling pathway; / >>Neurotrophin signaling pathway; / >>Adipocytokine signaling pathway; / >>Relaxin signaling pathway; / >>Insulin resistance; / >>Alcoholic liver disease; / >>Epithelial cell signaling in Helicobacter pylori infection; / >>Pathogenic Escherichia coli infection; / >>Shigellosis; / >>Salmonella infection; / >>Legionellosis; / >>Yersinia infection; / >>Leishmaniasis; / >>Chagas disease; / >>Toxoplasmosis; / >>Hepatitis C; / >>Hepatitis B; / >>Measles; / >>Human cytomegalovirus infection; / >>Influenza A; / >>Human T-cell leukemia virus 1 infection; / >>Kaposi sarcoma-associated herpesvirus infection; / >>Herpes simplex virus 1 infection; / >>Epstein-Barr virus infection; / >>Human immunodeficiency virus 1 infection; / >>Coronavirus disease - COVID-19; / >>Pathways in cancer; / >>Viral carcinogenesis; / >>Chemical carcinogenesis - reactive oxygen species; / >>Prostate cancer; / >>Chronic myeloid leukemia; / >>Small cell lung cancer; / >>PD-L1 expression and PD-1 checkpoint pathway in cancer; / >>Lipid and atherosclerosis |
| Function | Disease:Defects in NFKBIA are the cause of ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant (ADEDAID) [MIM:612132]. Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. ADEDAID is an ectodermal dysplasia associated with decreased production of pro-inflammatory cytokines and certain interferons, rendering patients susceptible to infection.,Function:Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to tranlocate to the nucleus and activate transcription.,induction:Induced in adherent monocytes.,online information:NFKBIA mutation db,PTM:Phosphorylated; disables inhibition of NF-kappa-B DNA-binding activity.,PTM:Sumoylated; sumoylation requires the presence of the nuclear import signal.,PTM:Ubiquitinated; subsequent to stimulus-dependent phosphorylation on serines.,similarity:Belongs to the NF-kappa-B inhibitor family.,similarity:Contains 5 ANK repeats.,subcellular location:Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export.,subunit:Interacts with RELA; the interaction requires the nuclear import signal. Interacts with NKIRAS1 and NKIRAS2. Part of a 70-90 kDa complex at least consisting of CHUK, IKBKB, NFKBIA, RELA, IKBKAP and MAP3K14. Interacts with HBV protein X. Interacts with RWDD3; the interaction enhances sumoylation., |
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