Name: IκB-β Polyclonal Antibody
SKU: WP12879
Price: 268 USD
Availability: InStock

Product details

Background:

The protein encoded by this gene belongs to the NF-kappa-B inhibitor family, which inhibit NF-kappa-B by complexing with, and trapping it in the cytoplasm. Phosphorylation of serine residues on these proteins by kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B, which translocates to the nucleus to function as a transcription factor. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jul 2011],

Specifications

Target	IκB β
Reactivity	Human, Mouse
Applications	WB, IHC
MW(Calculated)
MW(Observed)	37kD
Host Species	Rabbit
Isotype	IgG
Conjugate/Modification	Unmodified
Modification
Recommended Dilution Ratio	WB 1:500-2000; IHC 1:50-300 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#YS0004)
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration	1 mg/ml
Clonality	Polyclonal
Clone Number
Immunogen	The antiserum was produced against synthesized peptide derived from human IkappaB-beta. AA range:4-53
Sequence
Specificity	IκB-β Polyclonal Antibody detects endogenous levels of IκB-β protein.
Gene Name	NFKBIB
Protein Name	NF-kappa-B inhibitor beta
Other Name	NFKBIB; / IKBB; / TRIP9; / NF-kappa-B inhibitor beta; / NF-kappa-BIB; / I-kappa-B-beta; / IkB-B; / IkB-beta; / IkappaBbeta; / Thyroid receptor-interacting protein 9; / TR-interacting protein 9; / TRIP-9
Species	Human
Gene ID-1	4793
Uniprot	Q15653,
Species.1	Mouse
Gene ID-2	18036
Uniprot.1	Q60778
Species.2
Gene ID-3
Uniprot.2
Organism-4
Gene ID-4
SwissProt-4
Background	The protein encoded by this gene belongs to the NF-kappa-B inhibitor family, which inhibit NF-kappa-B by complexing with, and trapping it in the cytoplasm. Phosphorylation of serine residues on these proteins by kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B, which translocates to the nucleus to function as a transcription factor. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jul 2011],
Cellular Localization	Cytoplasm . Nucleus .
Tissue Expression	Expressed in all tissues examined.
Signaling_pathway	Organismal Systems >> Immune system >> NOD-like receptor signaling pathway;Organismal Systems >> Immune system >> RIG-I-like receptor signaling pathway;Organismal Systems >> Immune system >> Cytosolic DNA-sensing pathway;Organismal Systems >> Immune system >> T cell receptor signaling pathway;Organismal Systems >> Immune system >> Th1 and Th2 cell differentiation;Organismal Systems >> Immune system >> Th17 cell differentiation;Organismal Systems >> Immune system >> B cell receptor signaling pathway;Organismal Systems >> Immune system >> Chemokine signaling pathway;Organismal Systems >> Endocrine system >> Adipocytokine signaling pathway;Organismal Systems >> Nervous system >> Neurotrophin signaling pathway;Human Diseases >> Cancer: overview >> PD-L1 expression and PD-1 checkpoint pathway in cancer
Research Areas	>>Chemokine signaling pathway; / >>NOD-like receptor signaling pathway; / >>RIG-I-like receptor signaling pathway; / >>Cytosolic DNA-sensing pathway; / >>Th1 and Th2 cell differentiation; / >>Th17 cell differentiation; / >>T cell receptor signaling pathway; / >>B cell receptor signaling pathway; / >>Neurotrophin signaling pathway; / >>Adipocytokine signaling pathway; / >>Pathogenic Escherichia coli infection; / >>Shigellosis; / >>Leishmaniasis; / >>Toxoplasmosis; / >>Measles; / >>Influenza A; / >>Epstein-Barr virus infection; / >>Coronavirus disease - COVID-19; / >>PD-L1 expression and PD-1 checkpoint pathway in cancer
Function	Function:Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further IKBA-dependent inactivation. Association with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation rendering it more resistant to degradation, explaining its slower degradation.,PTM:Phosphorylated; followed by degradation. Interaction with NKIRAS1 and NKIRAS2 probably prevents phosphorylation.,similarity:Belongs to the NF-kappa-B inhibitor family.,similarity:Contains 6 ANK repeats.,subunit:Interacts with THRB (via ligand-binding domain). Interacts with RELA and REL. Interacts with COMMD1 and inhibitor kappa B-interacting Ras-like NKIRAS1 and NKIRAS2.,tissue specificity:Expressed in all tissues examined.,
RRID