Name: IFN-γ Polyclonal Antibody
SKU: WP12783
Price: 268 USD
Availability: InStock

Product details

Background:

This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Dec 2015],

Specifications

Size	50μL/100μL
Target	IFN-γ
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, ELISA
MW(Calculated)
MW(Observed)	17kD
Host Species	Rabbit
Isotype	IgG
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:40000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration	1 mg/ml
Clonality	Polyclonal
Clone Number
Immunogen	The antiserum was produced against synthesized peptide derived from human IFN-gamma. AA range:20-69
Specificity	IFN-γ Polyclonal Antibody detects endogenous levels of IFN-γ protein.
Gene Name	IFNG
Protein Name	Interferon gamma
Other Name	IFNG; / Interferon gamma; / IFN-gamma; / Immune interferon
Species	Human
Gene ID	3458
Uniprot	P01579,
Species.1	Mouse
Gene ID.1
Uniprot.1	P01580
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Secreted.
Tissue Expression	Released primarily from activated T lymphocytes.
Signaling_pathway	Cellular Processes >> Cell growth and death >> Necroptosis;Organismal Systems >> Immune system >> Natural killer cell mediated cytotoxicity;Organismal Systems >> Immune system >> Antigen processing and presentation;Organismal Systems >> Immune system >> T cell receptor signaling pathway;Organismal Systems >> Immune system >> Th1 and Th2 cell differentiation;Organismal Systems >> Immune system >> Th17 cell differentiation;Organismal Systems >> Immune system >> IL-17 signaling pathway;Organismal Systems >> Development and regeneration >> Osteoclast differentiation;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: overview >> PD-L1 expression and PD-1 checkpoint pathway in cancer;Human Diseases >> Immune disease >> Systemic lupus erythematosus;Human Diseases >> Immune disease >> Rheumatoid arthritis;Human Diseases >> Immune disease >> Inflammatory bowel disease;Human Diseases >> Immune disease >> Allograft rejection;Human Diseases >> Immune disease >> Graft-versus-host disease;Environmental Information Processing >> Signal transduction >> TGF-beta signaling pathway;Environmental Information Processing >> Signal transduction >> JAK-STAT signaling pathway;Environmental Information Processing >> Signal transduction >> HIF-1 signaling pathway;Environmental Information Processing >> Signaling molecules and interaction >> Cytokine-cytokine receptor interaction;Genetic Information Processing >> Folding, sorting and degradation >> Proteasome
Research Areas	>>Proteasome; / >>Cytokine-cytokine receptor interaction; / >>HIF-1 signaling pathway; / >>Necroptosis; / >>TGF-beta signaling pathway; / >>Osteoclast differentiation; / >>Antigen processing and presentation; / >>JAK-STAT signaling pathway; / >>Natural killer cell mediated cytotoxicity; / >>IL-17 signaling pathway; / >>Th1 and Th2 cell differentiation; / >>Th17 cell differentiation; / >>T cell receptor signaling pathway; / >>Type I diabetes mellitus; / >>Leishmaniasis; / >>Chagas disease; / >>African trypanosomiasis; / >>Malaria; / >>Toxoplasmosis; / >>Amoebiasis; / >>Tuberculosis; / >>Hepatitis C; / >>Influenza A; / >>Herpes simplex virus 1 infection; / >>Pathways in cancer; / >>PD-L1 expression and PD-1 checkpoint pathway in cancer; / >>Inflammatory bowel disease; / >>Systemic lupus erythematosus; / >>Rheumatoid arthritis; / >>Allograft rejection; / >>Graft-versus-host disease; / >>Fluid shear stress and atherosclerosis
Function	Disease:In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.,Function:Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.,online information:Interferon gamma entry,pharmaceutical:Available under the name Actimmune (Genentech). Used for reducing the frequency and severity of serious infections associated with chronic granulomatous disease (CGD).,PTM:Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.,similarity:Belongs to the type II (or gamma) interferon family.,subunit:Homodimer.,tissue specificity:Released primarily from activated T lymphocytes.,