Ikaros Monoclonal Antibody
Ucallm_WM0501-50
Price range: $268.00 through $328.00
Product details
Background:
This gene encodes a transcription factor that belongs to the family of zinc-finger DNA-binding proteins associated with chromatin remodeling. The expression of this protein is restricted to the fetal and adult hemo-lymphopoietic system, and it functions as a regulator of lymphocyte differentiation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. Most isoforms share a common C-terminal domain, which contains two zinc finger motifs that are required for hetero- or homo-dimerization, and for interactions with other proteins. The isoforms, however, differ in the number of N-terminal zinc finger motifs that bind DNA and in nuclear localization signal presence, resulting in members with and without DNA-binding properties. Only a few isoforms contain the requisite three or more N-terminal zinc motifs that confer high affinity binding
Specifications
| Size | 50μL/100μL |
| Target | Ikaros |
| Reactivity | Human, Mouse, Dog |
| Applications | WB |
| MW(Calculated) | 58kD |
| MW(Observed) | |
| Host Species | Mouse |
| Isotype | |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:1000-1:2000; Not yet tested in other applications. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | Affinity purification |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | 3C2 |
| Immunogen | Purified recombinant human Ikaros (C-terminus) protein fragments expressed in E.coli. |
| Specificity | Ikaros Monoclonal Antibody detects endogenous levels of Ikaros protein. |
| Gene Name | IKZF1 |
| Protein Name | DNA-binding protein Ikaros |
| Other Name | IKZF1; / IK1; / IKAROS; / LYF1; / ZNFN1A1; / DNA-binding protein Ikaros; / Ikaros family zinc finger protein 1; / Lymphoid transcription factor LyF-1 |
| Species | Human |
| Gene ID | 10320 |
| Uniprot | Q13422, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q03267 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nucleus . In resting lymphocytes, distributed diffusely throughout the nucleus. Localizes to pericentromeric heterochromatin in proliferating cells. This localization requires DNA binding which is regulated by phosphorylation / dephosphorylation events. .; [Isoform Ik2]: Nucleus. In resting lymphocytes, distributed diffusely throughout the nucleus. Localizes to pericentromeric heterochromatin in proliferating cells. This localization requires DNA binding which is regulated by phosphorylation / dephosphorylation events (By similarity). .; [Isoform Ik6]: Cytoplasm . |
| Tissue Expression | Abundantly expressed in thymus, spleen and peripheral blood Leukocytes and lymph nodes. Lower expression in bone marrow and small intestine. |
| Signaling_pathway | |
| Research Areas | |
| Function | Function:Binds and activates the enhancer (delta-A element) of the CD3-delta gene. Functions in the specification and the maturation of the T-lymphocyte. Also interacts with a critical control element in the TDT (terminal deoxynucleotidyltransferase) promoter as well as with the promoters for other genes expressed during early stages of B- and T-cell development.,similarity:Belongs to the Ikaros C2H2-type zinc-finger protein family.,similarity:Contains 6 C2H2-type zinc fingers.,subunit:Interacts with IKZF4 AND IKZF5.,tissue specificity:Abundantly expressed in thymus, spleen and peripheral blood Leukocytes and lymph nodes. Lower expression in bone marrow and small intestine., |
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