Price range: $268.00 through $328.00
Background:
IKBKE is a noncanonical I-kappa-B (see MIM 164008) kinase (IKK) that is essential for regulating antiviral signaling pathways. IKBKE has also been identified as a breast cancer (MIM 114480) oncogene and is amplified and overexpressed in over 30% of breast carcinomas and breast cancer cell lines (Hutti et al., 2009 [PubMed 19481526]).[supplied by OMIM, Oct 2009],
| Size | 50μL/100μL |
| Target | IKKε |
| Reactivity | Human |
| Applications | WB, ELISA |
| MW(Calculated) | 80kD |
| MW(Observed) | |
| Host Species | Mouse |
| Isotype | |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; ELISA 1:10000; Not yet tested in other applications. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | Affinity purification |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | 13C9 |
| Immunogen | Purified recombinant fragment of IKKε (aa1-257) expressed in E. Coli. |
| Specificity | IKKε Monoclonal Antibody detects endogenous levels of IKKε protein. |
| Gene Name | IKBKE IKKE IKKI KIAA0151 |
| Protein Name | Inhibitor of nuclear factor kappa-B kinase subunit epsilon |
| Other Name | IKBKE; / IKKE; / IKKI; / KIAA0151; / Inhibitor of nuclear factor kappa-B kinase subunit epsilon; / I-kappa-B kinase epsilon; / IKK-E; / IKK-epsilon; / IkBKE; / Inducible I kappa-B kinase; / IKK-i |
| Species | Human |
| Gene ID | 9641 |
| Uniprot | Q14164 |
| Species.1 | |
| Gene ID.1 | |
| Uniprot.1 | |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasm . Nucleus. Nucleus, PML body . Targeting to PML nuclear bodies upon DNA damage is TOPORS-dependent (PubMed:20188669). Located diffusely throughout the cytoplasm but locates to punctate cytoplasmic bodies when coexpressed with TRIM6 (PubMed:24882218). . |
| Tissue Expression | Highly expressed in spleen followed by thymus, peripheral blood leukocytes, pancreas, placenta. Weakly expressed in lung, kidney, prostate, ovary and colon. |
| Signaling_pathway | Organismal Systems >> Immune system >> Toll-like receptor signaling pathway;Organismal Systems >> Immune system >> NOD-like receptor signaling pathway;Organismal Systems >> Immune system >> RIG-I-like receptor signaling pathway;Organismal Systems >> Immune system >> Cytosolic DNA-sensing pathway;Organismal Systems >> Immune system >> IL-17 signaling pathway |
| Research Areas | >>Toll-like receptor signaling pathway; / >>NOD-like receptor signaling pathway; / >>RIG-I-like receptor signaling pathway; / >>Cytosolic DNA-sensing pathway; / >>C-type lectin receptor signaling pathway; / >>IL-17 signaling pathway; / >>Alcoholic liver disease; / >>Hepatitis C; / >>Hepatitis B; / >>Measles; / >>Influenza A; / >>Human papillomavirus infection; / >>Kaposi sarcoma-associated herpesvirus infection; / >>Herpes simplex virus 1 infection; / >>Epstein-Barr virus infection; / >>Coronavirus disease - COVID-19; / >>Chemical carcinogenesis - receptor activation; / >>Lipid and atherosclerosis |
| Function | Catalytic activity:ATP + [I-kappa-B protein] = ADP + [I-kappa-B phosphoprotein].,Function:Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. May play a special role in the immune response.,PTM:Autophosphorylated.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.,similarity:Contains 1 protein kinase domain.,subunit:May interact with MAVS/IPS1. Interacts with AZI2. Interacts with SIKE. Interacts with TICAM1/TRIF, IRF3 and DDX58/RIG-I, interactions are disrupted by the interaction between IKBKE and SIKE.,tissue specificity:Highly expressed in spleen followed by thymus, peripheral blood leukocytes, pancreas, placenta. Weakly expressed in lung, kidney, prostate, ovary and colon., |
