IL-1β Polyclonal Antibody
Ucallm_WP15277-50
Price range: $268.00 through $328.00
Product details
Background:
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008],
Specifications
| Size | 50μL/100μL |
| Target | IL-1β |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 17kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC: 1:100-300; ELISA 1:20000; IF 1:100-300; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human IL1B. AA range:181-230 |
| Specificity | IL-1β Polyclonal Antibody detects endogenous levels of IL-1β protein. |
| Gene Name | IL1B IL1F2 |
| Protein Name | Interleukin-1 beta |
| Other Name | IL1B; / IL1F2; / Interleukin-1 beta; / IL-1 beta; / Catabolin |
| Species | Human |
| Gene ID | 3553 |
| Uniprot | P01584, |
| Species.1 | Mouse |
| Gene ID.1 | 16176 |
| Uniprot.1 | P10749, |
| Species.2 | Rat |
| Gene ID.2 | |
| Uniprot.2 | Q63264 |
| Cellular Localization | Cytoplasm, cytosol . Secreted . Lysosome . Secreted, extracellular exosome . The precursor is cytosolic (PubMed:15192144). In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted (PubMed:24201029, PubMed:33377178, PubMed:33883744). Mature form is secreted and released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore (PubMed:33883744). In contrast, the precursor form is not released, due to the presence of an acidic region that is proteolytically removed by CASP1 during maturation (PubMed:33883744). The secretion is dependent on protein unfolding and facilitated by the cargo receptor TMED10 (PubMed:32272059). . |
| Tissue Expression | Expressed in activated monocytes/macrophages (at protein level). |
| Signaling_pathway | Cellular Processes >> Cell growth and death >> Necroptosis;Organismal Systems >> Immune system >> Hematopoietic cell lineage;Organismal Systems >> Immune system >> Toll-like receptor signaling pathway;Organismal Systems >> Immune system >> NOD-like receptor signaling pathway;Organismal Systems >> Immune system >> Cytosolic DNA-sensing pathway;Organismal Systems >> Immune system >> Th17 cell differentiation;Organismal Systems >> Immune system >> IL-17 signaling pathway;Organismal Systems >> Sensory system >> Inflammatory mediator regulation of TRP channels;Organismal Systems >> Development and regeneration >> Osteoclast differentiation;Human Diseases >> Immune disease >> Rheumatoid arthritis;Human Diseases >> Immune disease >> Inflammatory bowel disease;Human Diseases >> Immune disease >> Graft-versus-host disease;Human Diseases >> Neurodegenerative disease >> Alzheimer disease;Human Diseases >> Neurodegenerative disease >> Prion disease;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases;Environmental Information Processing >> Signal transduction >> MAPK signaling pathway;Environmental Information Processing >> Signal transduction >> NF-kappa B signaling pathway;Environmental Information Processing >> Signal transduction >> TNF signaling pathway;Environmental Information Processing >> Signaling molecules and interaction >> Cytokine-cytokine receptor interaction |
| Research Areas | >>Antifolate resistance; / >>MAPK signaling pathway; / >>Cytokine-cytokine receptor interaction; / >>NF-kappa B signaling pathway; / >>Necroptosis; / >>Osteoclast differentiation; / >>Toll-like receptor signaling pathway; / >>NOD-like receptor signaling pathway; / >>Cytosolic DNA-sensing pathway; / >>C-type lectin receptor signaling pathway; / >>Hematopoietic cell lineage; / >>IL-17 signaling pathway; / >>Th17 cell differentiation; / >>TNF signaling pathway; / >>Inflammatory mediator regulation of TRP channels; / >>Non-alcoholic fatty liver disease; / >>AGE-RAGE signaling pathway in diabetic complications; / >>Alcoholic liver disease; / >>Type I diabetes mellitus; / >>Alzheimer disease; / >>Prion disease; / >>Pathways of neurodegeneration - multiple diseases; / >>Pathogenic Escherichia coli infection; / >>Shigellosis; / >>Salmonella infection; / >>Pertussis; / >>Legionellosis; / >>Yersinia infection; / >>Leishmaniasis; / >>Chagas disease; / >>African trypanosomiasis; / >>Malaria; / >>Amoebiasis; / >>Tuberculosis; / >>Measles; / >>Human cytomegalovirus infection; / >>Influenza A; / >>Herpes simplex virus 1 infection; / >>Coronavirus disease - COVID-19; / >>Inflammatory bowel disease; / >>Rheumatoid arthritis; / >>Graft-versus-host disease; / >>Lipid and atherosclerosis; / >>Fluid shear stress and atherosclerosis |
| Function | Domain:The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.,Function:Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.,online information:Interleukin-1 entry,online information:The Singapore human mutation and polymorphism database,similarity:Belongs to the IL-1 family.,subcellular location:The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.,subunit:Monomer., |
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