Name: Integrin α6 Monoclonal Antibody
SKU: WM0503
Price: 268 USD
Availability: InStock

Product details

Background:
integrin subunit alpha 6(ITGA6) Homo sapiens The gene encodes a member of the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 6 subunit. This subunit may associate with a beta 1 or beta 4 subunit to form an integrin that interacts with extracellular matrix proteins including members of the laminin family. The alpha 6 beta 4 integrin may promote tumorigenesis, while the alpha 6 beta 1 integrin may negatively regulate erbB2/HER2 signaling. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015],

Specifications

Size	50μL/100μL
Target	Integrin α6
Reactivity	Human, Mouse, Rat, Bovine, Dog, Pig
Applications	WB, IF
MW(Calculated)	127kD
MW(Observed)
Host Species	Mouse
Isotype
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	WB 1:1000-1:2000; IF 1:100-1:500; Not yet tested in other applications.
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification	Affinity purification
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	7F10
Immunogen	Purified recombinant human Integrin α6 (N-terminus) protein fragments expressed in E.coli.
Specificity	Integrin α6 Monoclonal Antibody detects endogenous levels of Integrin α6 protein.
Gene Name	ITGA6
Protein Name	Integrin alpha-6
Other Name	ITGA6; / Integrin alpha-6; / CD49 antigen-like family member F; / VLA-6; / CD antigen CD49f
Species	Human
Gene ID	3655
Uniprot	P23229,
Species.1	Mouse
Gene ID.1
Uniprot.1	Q61739
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Cell membrane; Single-pass type I membrane protein . Cell membrane; Lipid-anchor .
Tissue Expression	Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoforms containing segment X1 are ubiquitously expressed. Isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and in a limited number of cell lines; they are always coexpressed with the ubiquitous isoform containing segment X1. In some tissues (e.g. Salivary gland), isoforms containing cytoplasmic segment A and isoforms containing segment B are detected while in others, only isoforms containing one cytoplasmic segment are found (segment A in epidermis and segment B in kidney). Processed integrin alpha-6: Expressed at low levels in normal prostate tissue with elevated levels in prostate cancer tissue (at protein level) (PubMed:15023541).
Signaling_pathway	Cellular Processes >> Cellular community - eukaryotes >> Focal adhesion;Cellular Processes >> Cell motility >> Regulation of actin cytoskeleton;Organismal Systems >> Immune system >> Hematopoietic cell lineage;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: specific types >> Small cell lung cancer;Environmental Information Processing >> Signal transduction >> PI3K-Akt signaling pathway;Environmental Information Processing >> Signaling molecules and interaction >> ECM-receptor interaction;Environmental Information Processing >> Signaling molecules and interaction >> Cell adhesion molecules
Research Areas	>>PI3K-Akt signaling pathway; / >>Focal adhesion; / >>ECM-receptor interaction; / >>Cell adhesion molecules; / >>Hematopoietic cell lineage; / >>Regulation of actin cytoskeleton; / >>Toxoplasmosis; / >>Human papillomavirus infection; / >>Pathways in cancer; / >>Small cell lung cancer; / >>Hypertrophic cardiomyopathy; / >>Arrhythmogenic right ventricular cardiomyopathy; / >>Dilated cardiomyopathy
Function	Alternative products:Additional isoforms seem to exist. There is a combination of at least four alternatively spliced domains, two extracellular (X1 and X2) and two cytoplasmic (A and B). So far detected are isoform Alpha-6X1A, isoform Alpha-6X1B and isoform Alpha-6X1X2A (minor). Experimental confirmation may be lacking for some isoforms,Disease:Defects in ITGA6 are a cause of epidermolysis bullosa with pyloric atresia (EB-PA) [MIM:226730]; also known as aplasia cutis congenita with gastrointestinal atresia. EB-PA is an autosomal recessive disease characterized by mucocutaneous fragility and gastrointestinal atresia, which most commonly affects the pylorus.,Function:Integrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome.,PTM:Isoforms containing segment A, but not segment B, are the major targets for PMA-induced phosphorylation. Phosphorylation occurs on 'Ser-1103' of isoform alpha-6X1X2A. Phosphorylation is not required for the induction of integrin alpha-6A/beta-1 high affinity but may reduce the affinity for ligand.,similarity:Belongs to the integrin alpha chain family.,similarity:Contains 7 FG-GAP repeats.,subunit:Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-6 associates with either beta-1 or beta-4. Interacts with HPS5. Interacts with RAB21.,tissue specificity:Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoforms containing segment X1 are ubiquitously expressed. Isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and in a limited number of cell lines; they are always coexpressed with the ubiquitous isoform containing segment X1. In some tissues (e.g. Salivary gland), isoforms containing cytoplasmic segment A and isoforms containing segment B are detected while in others, only isoforms containing one cytoplasmic segment are found (segment A in epidermis and segment B in kidney).,