Price range: $268.00 through $328.00
Background:
This gene encodes a zinc finger protein that contains a jumonji domain and may play a role in hormone-dependent transcriptional activation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009],
| Size | 50μL/100μL |
| Target | JMJD1A |
| Reactivity | Human |
| Applications | WB, IF, ELISA |
| MW(Calculated) | 147kD |
| MW(Observed) | |
| Host Species | Mouse |
| Isotype | |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IF 1:200-1:1000; ELISA 1:10000; Not yet tested in other applications. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | Affinity purification |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | 1C7 |
| Immunogen | Purified recombinant fragment of human JMJD1A expressed in E. Coli. |
| Specificity | JMJD1A Monoclonal Antibody detects endogenous levels of JMJD1A protein. |
| Gene Name | KDM3A |
| Protein Name | Lysine-specific demethylase 3A |
| Other Name | KDM3A; / JHDM2A; / JMJD1; / JMJD1A; / KIAA0742; / TSGA; / Lysine-specific demethylase 3A; / JmjC domain-containing histone demethylation protein 2A; / Jumonji domain-containing protein 1A |
| Species | Human |
| Gene ID | 55818 |
| Uniprot | Q9Y4C1, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q6PCM1 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasm . Nucleus . Nuclear in round spermatids. When spermatids start to elongate, localizes to the cytoplasm where it forms distinct foci which disappear in mature spermatozoa (By similarity). . |
| Tissue Expression | Adrenal gland,Brain,Fetal kidney,Salivary gland,Testis, |
| Signaling_pathway | |
| Research Areas | >>Thermogenesis |
| Function | cofactor:Binds 1 Fe(2+) ion per subunit.,Domain:Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs are known to mediate the association with nuclear receptors.,Domain:The JmjC domain and the C6-type zinc-finger are required for the demethylation activity.,Function:Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.,similarity:Belongs to the JHDM2 histone demethylase family.,similarity:Contains 1 JmjC domain.,subcellular location:Nuclear in round spermatids. When spermatids start to elongate, localizes to the cytoplasm where it forms distinct foci which disappear in mature spermatozoa., |
