Name: KAP1/TIF1β mouse mAb
SKU: WM0578
Price: 268 USD
Availability: InStock

Product details

Background:
The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq, Jul 2008],

Specifications

Target	TIF1β
Reactivity	Human
Applications	WB, ICC, IHC, IP
MW(Calculated)
MW(Observed)	110kD
Host Species	Mouse
Isotype
Conjugate/Modification	Unmodified
Modification
Recommended Dilution Ratio	WB 1:1000; ICC 1:100
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Purification	The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Purity
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	9H12
Immunogen	Purified recombinant human KAP1 / TIF1 beta protein fragments expressed in E.coli.
Sequence
Specificity	This antibody detects endogenous levels of KAP1 / TIF1 beta and does not cross-react with related proteins.
Gene Name	trim28
Protein Name
Other Name	E3 SUMO protein ligase TRIM28; / E3 SUMO-protein ligase TRIM28; / FLJ29029; / KAP 1; / KAP-1; / KRAB associated protein 1; / KRAB interacting protein 1; / KRAB-associated protein 1; / KRAB-interacting protein 1; / KRIP 1; / KRIP-1; / KRIP1; / Nuclear corepressor KAP 1; / Nuclear corepressor KAP-1; / RING finger protein 96; / RNF96; / TF1B; / TIF1 beta; / TIF1-beta; / TIF1B; / TIF1B_HUMAN; / Transcription intermediary factor 1 beta; / Transcription intermediary factor 1-beta; / TRIM28; / Tripartite motif containing 28; / tripartite motif containing protein 28; / Tripartite motif-containing protein 28.
Species	Human
Gene ID-1	10155
Uniprot	Q13263,
Species.1	Mouse
Gene ID-2	21849
Uniprot.1	Q62318
Species.2
Gene ID-3
Uniprot.2
Organism-4
Gene ID-4
SwissProt-4
Background	The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq, Jul 2008],
Cellular Localization	Nucleus . Associated with centromeric heterochromatin during cell differentiation through CBX1. .
Tissue Expression	Expressed in all tissues tested including spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes.
Signaling_pathway
Research Areas
Function	Domain:Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.,Domain:The HP1 box is both necessary and sufficient for HP1 binding. The RING finger domain and the B-box domains mediate interaction with CEBPB. The PHD domain enhances the CEBPB transcriptional activity.,Function:Forms a complex with a KRAB-domain transcription factor and increases the efficiency of KRAB-mediated repression. Silences transcription through an interaction with HP1 proteins. Acts as a corepressor of transcription for the KRAB zinc finger proteins and as a moderator of the repression activity. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of the Alpha-1-acid glycoprotein gene.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the TRIM/RBCC family.,similarity:Contains 1 bromo domain.,similarity:Contains 1 PHD-type zinc finger.,similarity:Contains 1 RING-type zinc finger.,similarity:Contains 2 B box-type zinc fingers.,subcellular location:Associated with centromeric heterochromatin during cell differentiation through CBX1.,subunit:Associated with HP1 alpha (CBX5), beta (CBX1) and gamma (CBX3) in interphase nuclei (By similarity). Interacts with ZNF382; enhances ZNF382 transcriptional repressor activity (By similarity). Interacts with CEBPB and NR3C1 (By similarity). Interacts with NCOR1 and CHD3. Interacts with SETDB1, ZFP53 and ZFP68. Interacts directly with CBX5 via the PxVxL motif.,
RRID