U-Blot® KLF4 Rabbit mAb
WR4081
WR4081 Immunohistochemical analysis of paraffin-embedded rat colon tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
WR4081 Immunohistochemical analysis of paraffin-embedded human colon tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
WR4081 Immunohistochemical analysis of paraffin-embedded mouse colon tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
WR4081 Western blot analysis of lysates from HCT-116 cells, using KLF4 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (Goat anti-Rabbit IgG antibody ) was used to detect the antibody.
Price range: $268.00 through $328.00
Product details
Background:This gene encodes a protein that belongs to the Kruppel family of transcription factors. The encoded zinc finger protein is required for normal development of the barrier function of skin. The encoded protein is thought to control the G1-to-S transition of the cell cycle following DNA damage by mediating the tumor suppressor gene p53. Mice lacking this gene have a normal appearance but lose weight rapidly, and die shortly after birth due to fluid evaporation resulting from compromised epidermal barrier function. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015],
Specifications
| Size | 50μL/100μL |
| Target | KLF4 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 55kD |
| MW(Observed) | 60kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1000; WB 1:1000-5000; IF 1:200-1000; ELISA 1:5000-20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0167R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | KLF4 |
| Protein Name | Krueppel-like factor 4 |
| Other Name | KLF4; / EZF; / GKLF; / Krueppel-like factor 4; / Epithelial zinc finger protein EZF; / Gut-enriched krueppel-like factor |
| Species | Human |
| Gene ID | 9314 |
| Uniprot | O43474, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q60793 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nuclear |
| Tissue Expression | Cervix,Lung,Placenta,Substantia nigra,Tongue, |
| Signaling_pathway | Cellular Processes >> Cellular community - eukaryotes >> Signaling pathways regulating pluripotency of stem cells |
| Research Areas | >>Signaling pathways regulating pluripotency of stem cells; / >>Chemical carcinogenesis - receptor activation |
| Function | Function:Transcription factor which acts as both an activator and repressor. Binds the CACCC core sequence. Binds to multiple sites in the 5'-flanking region of its own gene and can activate its own transcription. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development.,similarity:Belongs to the krueppel C2H2-type zinc-finger protein family.,similarity:Contains 3 C2H2-type zinc fingers.,subunit:Interaction with the C-terminal domain of MUC1 enhances suppression of TP53/p53 transcription., |
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