Name: Ku80 mouse mAb
SKU: WM0703
Price: 268 USD
Availability: InStock

Product details

Background:
The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq, Jul 2008],

Specifications

Size	50μL/100μL
Target	Ku-80
Reactivity	Human, Monkey
Applications	WB, IF, IP
MW(Calculated)
MW(Observed)	86kD
Host Species	Mouse
Isotype
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	WB 1:1000; ICC 1:400; IP 1:100; IF 1:50-200
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification	The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	14D7
Immunogen	Purified recombinant human Ku80 protein fragments expressed in E.coli
Specificity	This antibody detects endogenous levels of Ku80 and does not cross-react with related proteins.
Gene Name	xrcc5
Protein Name
Other Name	ATP dependent DNA helicase II 80 kDa subunit; / ATP dependent DNA helicase II 86 Kd subunit; / ATP dependent DNA helicase II; / ATP-dependent DNA helicase 2 subunit 2; / ATP-dependent DNA helicase II 80 kDa subunit; / CTC box binding factor 85 kDa; / CTC box-binding factor 85 kDa subunit; / CTC85; / CTCBF; / DNA repair protein XRCC5; / Double strand break rejoining; / FLJ39089; / G22P2; / KARP 1; / KARP1; / Ku 80; / Ku autoantigen 80kDa; / Ku80; / Ku86; / Ku86 autoantigen related protein 1; / KUB 2; / KUB2; / Lupus Ku autoantigen protein p86; / NFIV; / Nuclear factor IV; / Thyroid lupus autoantigen; / Thyroid-lupus autoantigen; / TLAA; / X ray repair complementing defective repair in Chinese hamster cells 5; / double strand break rejoining; / X-ray repair complementing defective repair in Chinese hamster cells 5; / double-strand-break rejoining; / X-ray repair cross-complementing protein 5; / Xray repair complementing defective repair in Chinese hamster cells 5; / XRCC 5; / XRCC5; / XRCC5_HUMAN.
Species	Human
Gene ID	7520
Uniprot	P13010,
Species.1	Mouse
Gene ID.1
Uniprot.1	P27641
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Nucleus . Nucleus, nucleolus . Chromosome .
Tissue Expression	Cervix carcinoma,Coronary artery,Heart,Neuroblastoma,Osteoblast,Thy
Signaling_pathway
Research Areas	>>Non-homologous end-joining
Function	developmental stage:Expression increases during promyelocyte differentiation.,Disease:Individuals with systemic lupus erythematosus (SLE) and related disorders produce extremely large amounts of autoantibodies to p70 and p86.,Domain:The EEXXXDDL motif is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage.,Function:Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by p70. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The Ku p70/p86 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The Ku p70/p86 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NARG1, the Ku p70/p86 dimer binds to the osteocalcin promoter and activates osteocalcin expression.,induction:In osteoblasts, by FGF2.,PTM:Phosphorylated on serine residues. Phosphorylation by PRKDC may enhance helicase activity.,PTM:Sumoylated.,similarity:Belongs to the ku80 family.,similarity:Contains 1 Ku domain.,subunit:Heterodimer of a 70 kDa and a 80 kDa subunit. The dimer associates in a DNA-dependent manner with PRKDC to form the DNA-dependent protein kinase complex DNA-PK, and with the LIG4-XRCC4 complex. The dimer also associates with NARG1, and this complex displays DNA binding activity towards the osteocalcin FGF response element (OCFRE). In addition, the 80 kDa subunit binds to the osteoblast-specific transcription factors MSX2 and RUNX2. Interacts with ELF3. May interact with APLF.,

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Ku80 mouse mAb

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Ku80 mouse mAb

Product details

Specifications

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